Colloidal gold particles, 25 to 55 A in diameter, which had been coated with polyvinylpyrrolidone, were microinjected into the ground cytoplasm of amebas (Chaos chaos). At time intervals of 1 minute, 2 minutes, 10 minutes, and 24 hours after injection the cells were fixed for electron microscopy. After 24 hours, gold particles were found in both the nuclei and the ground cytoplasm, the concentration being higher in the nuclei. Colloidal particles were also present in the nuclei after 10 minutes, but at this time interval the concentration did not appear to be greater than that in the ground cytoplasm. One and 2 minutes after injection, the gold particles were located almost exclusively in the ground cytoplasm; however, individual particles were often found within the annuli of the nuclear envelope, and were located specifically in the centers of these structures. The results suggest that at least some of the gold particles which enter the nuclei pass through the annuli, and that passage through these structures may be restricted to a central channel.
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Article| July 01 1962
THE NUCLEAR ANNULI AS PATHWAYS FOR NUCLEOCYTOPLASMIC EXCHANGES
Carl M. Feldherr
From the Department of Anatomy, School of Medicine, University of Pennsylvania, Philadelphia.
Dr. Feldherr's present address is Department of Physiology, University of Alberta, Edmonton, Alberta, Canada
Received: February 27 1962
Online Issn: 1540-8140
Print Issn: 0021-9525
Copyright, 1962, by The Rockefeller Institute Press
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Carl M. Feldherr; THE NUCLEAR ANNULI AS PATHWAYS FOR NUCLEOCYTOPLASMIC EXCHANGES . J Cell Biol 1 July 1962; 14 (1): 65–72. doi: https://doi.org/10.1083/jcb.14.1.65
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