The role of the Drosophila lamin protein in nuclear envelope assembly was studied using a Drosophila in vitro assembly system that reconstitutes nuclei from added sperm chromatin or naked DNA. Upon incubation of the embryonic assembly extract with anti-Drosophila lamin antibodies, the attachment of nuclear membrane vesicles to chromatin surface and nuclear envelope formation did not occur. Lamina assembly and nuclear membrane vesicles attachment to the chromatin were inhibited only when the activity of the 75-kD lamin isoform was inhibited in both soluble and membrane-vesicles fractions. Incubation of decondensed sperm chromatin with an extract that was depleted of nuclear membranes revealed the presence of lamin molecules on the chromatin periphery. In addition, high concentrations of bacterially expressed lamin molecules added to the extract, were able to associate with the chromatin periphery, and did not inhibit nuclear envelope assembly. After nuclear reconstitution, a fraction of the lamin pool was converted into the typical 74- and 76-kD isoforms. Together, these data strongly support an essential role of the lamina in nuclear envelope assembly.
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October 01 1992
Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract.
N Ulitzur,
N Ulitzur
Department of Genetics, Hebrew University of Jerusalem, Israel.
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A Harel,
A Harel
Department of Genetics, Hebrew University of Jerusalem, Israel.
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N Feinstein,
N Feinstein
Department of Genetics, Hebrew University of Jerusalem, Israel.
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Y Gruenbaum
Y Gruenbaum
Department of Genetics, Hebrew University of Jerusalem, Israel.
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N Ulitzur
Department of Genetics, Hebrew University of Jerusalem, Israel.
A Harel
Department of Genetics, Hebrew University of Jerusalem, Israel.
N Feinstein
Department of Genetics, Hebrew University of Jerusalem, Israel.
Y Gruenbaum
Department of Genetics, Hebrew University of Jerusalem, Israel.
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1992) 119 (1): 17–25.
Citation
N Ulitzur, A Harel, N Feinstein, Y Gruenbaum; Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract.. J Cell Biol 1 October 1992; 119 (1): 17–25. doi: https://doi.org/10.1083/jcb.119.1.17
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