High efficiency gene transfer (greater than 90%) in chicken dorsal root ganglion neurons has been obtained by DNA calcium phosphate co-precipitation, hence providing an important tool to study control of gene expression in primary neurons. Transfection with c-fos promoter sequences linked to the chloramphenicol acetyltransferase reporter gene showed that the serum responsive element functions as a strong transcriptional enhancer. Transcription from this element is developmentally regulated, and mediates the genetic response to nerve growth factor (NGF) in developing avian sensory neurons. Furthermore, NGF exerts a negative effect on transcription from the cyclic AMP responsive element, thereby supporting the involvement of tyrosine kinase activation by NGF in primary sensory neurons.
Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons.
N Gabellini, M C Minozzi, A Leon, R Dal Toso; Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons.. J Cell Biol 1 July 1992; 118 (1): 131–138. doi: https://doi.org/10.1083/jcb.118.1.131
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