The firefly luciferase protein contains a peroxisomal targeting signal at its extreme COOH terminus (Gould et al., 1987). Site-directed mutagenesis of the luciferase gene reveals that this peroxisomal targeting signal consists of the COOH-terminal three amino acids of the protein, serine-lysine-leucine. When this tripeptide is appended to the COOH terminus of a cytosolic protein (chloramphenicol acetyltransferase), it is sufficient to direct the fusion protein into peroxisomes. Additional mutagenesis experiments reveal that only a limited number of conservative changes can be made in this tripeptide targeting signal without abolishing its activity. These results indicate that peroxisomal protein import, unlike other types of transmembrane translocation, is dependent upon a conserved amino acid sequence.
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May 01 1989
A conserved tripeptide sorts proteins to peroxisomes.
S J Gould,
S J Gould
Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093.
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G A Keller,
G A Keller
Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093.
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N Hosken,
N Hosken
Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093.
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J Wilkinson,
J Wilkinson
Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093.
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S Subramani
S Subramani
Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093.
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S J Gould
Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093.
G A Keller
Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093.
N Hosken
Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093.
J Wilkinson
Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093.
S Subramani
Department of Biology and Center for Molecular Genetics, University of California, La Jolla, California 92093.
Online Issn: 1540-8140
Print Issn: 0021-9525
J Cell Biol (1989) 108 (5): 1657–1664.
Citation
S J Gould, G A Keller, N Hosken, J Wilkinson, S Subramani; A conserved tripeptide sorts proteins to peroxisomes.. J Cell Biol 1 May 1989; 108 (5): 1657–1664. doi: https://doi.org/10.1083/jcb.108.5.1657
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