The capacity of the centrosome to influence the lattice structure of nucleated microtubules was studied in vitro. Brain microtubules self-assembled to give predominantly (98%) 14-protofilament microtubules. However, under exactly the same conditions of assembly they grew off of purified centrosomes from neuroblastoma cells to give mostly (82%) 13-protofilament microtubules. Thus, the nucleation sites on the centrosome constrained the microtubule lattice to yield the number of protofilaments usually found in vivo.
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Article| April 01 1985
Influence of the centrosome on the structure of nucleated microtubules.
In Special Collection: JCB65: Cell Division, Cell Cycle, and Polarity
Online ISSN: 1540-8140
Print ISSN: 0021-9525
J Cell Biol (1985) 100 (4): 1185–1191.
L Evans, T Mitchison, M Kirschner; Influence of the centrosome on the structure of nucleated microtubules.. J Cell Biol 1 April 1985; 100 (4): 1185–1191. doi: https://doi.org/10.1083/jcb.100.4.1185
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