The intricacy of nuclear pore complex (NPC) biogenesis imposes risks of failure that can cause defects in nuclear transport and nuclear envelope (NE) morphology; however, cellular mechanisms used to alleviate NPC assembly stress are not well defined. In the budding yeast Saccharomyces cerevisiae, we demonstrate that NVJ1- and MDM1-enriched NE–vacuole contacts increase when NPC assembly is compromised in several nup mutants, including nup116ΔGLFG cells. These interorganelle nucleus–vacuole junctions (NVJs) cooperate with lipid droplets to maintain viability and enhance NPC formation in assembly mutants. Additionally, NVJs function with ATG1 to remodel the NE and promote vacuole-dependent degradation of specific nucleoporins in nup116ΔGLFG cells. Importantly, NVJs significantly improve the physiology of NPC assembly mutants, despite having only negligible effects when NPC biogenesis is unperturbed. These results therefore define how NE–vacuole interorganelle contacts coordinate responses to mitigate deleterious cellular effects caused by disrupted NPC assembly.
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7 December 2020
Article|
October 14 2020
Nuclear envelope–vacuole contacts mitigate nuclear pore complex assembly stress
Christopher L. Lord,
Christopher L. Lord
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN
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Susan R. Wente
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN
Correspondence to Susan R. Wente: susan.wente@vanderbilt.edu
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Christopher L. Lord
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN
Susan R. Wente
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN
Correspondence to Susan R. Wente: susan.wente@vanderbilt.edu
Received:
January 29 2020
Revision Received:
August 10 2020
Accepted:
September 23 2020
Online Issn: 1540-8140
Print Issn: 0021-9525
© 2020 Lord and Wente
2020
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
J Cell Biol (2020) 219 (12): e202001165.
Article history
Received:
January 29 2020
Revision Received:
August 10 2020
Accepted:
September 23 2020
Citation
Christopher L. Lord, Susan R. Wente; Nuclear envelope–vacuole contacts mitigate nuclear pore complex assembly stress. J Cell Biol 7 December 2020; 219 (12): e202001165. doi: https://doi.org/10.1083/jcb.202001165
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