Aurora B kinase is essential for faithful chromosome segregation during mitosis. During (pro)metaphase, Aurora B is concentrated at the inner centromere by the kinases Haspin and Bub1. However, how Haspin and Bub1 collaborate to control Aurora B activity at centromeres remains unclear. Here, we show that either Haspin or Bub1 activity is sufficient to recruit Aurora B to a distinct chromosomal locus. Moreover, we identified a small, Bub1 kinase–dependent Aurora B pool that supported faithful chromosome segregation in otherwise unchallenged cells. Joined inhibition of Haspin and Bub1 activities fully abolished Aurora B accumulation at centromeres. While this impaired the correction of erroneous KT–MT attachments, it did not compromise the mitotic checkpoint, nor the phosphorylation of the Aurora B kinetochore substrates Hec1, Dsn1, and Knl1. This suggests that Aurora B substrates at the kinetochore are not phosphorylated by centromere-localized pools of Aurora B, and calls for a reevaluation of the current spatial models for how tension affects Aurora B–dependent kinetochore phosphorylation.
Untangling the contribution of Haspin and Bub1 to Aurora B function during mitosis
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Michael A. Hadders, Sanne Hindriksen, My Anh Truong, Aditya N. Mhaskar, J. Pepijn Wopken, Martijn J.M. Vromans, Susanne M.A. Lens; Untangling the contribution of Haspin and Bub1 to Aurora B function during mitosis. J Cell Biol 2 March 2020; 219 (3): e201907087. doi: https://doi.org/10.1083/jcb.201907087
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