Ribosomal RNAs (28 + 18S and 5S) and 4S RNA extracted from the chironomid Glyptotendipes barbipes were iodinated in vitro with 125I and hybridized to the salivary gland chromosomes of G. barbipes and Drosophila melanogaster. Iodinated 18 + 28 S RNA labeled three puffed sites with associated nucleoli on chromosomes IR, IIL, and IIIL of G. barbipes and the nucleolar organizer of Drosophila. Labeled 5S RNA hybridized to three sites on chromosome IIIR, two sites on chromosome IIR and one site in a Balbiani ring on chromosome IV of Glyptotendipes. Most of the label produced by this RNA was localized seven bands away from the centromere on the right arm of chromosome III, and we consider this to be the main site complementary to 5S RNA in the chironomid. This same RNA preparation specifically labeled the 56 EF region of chromosome IIR of Drosophila which has been shown previously to be the only site labeled when hybridized with homologous 5S RNA. Hybridization of G. barbipes chromosomes with iodinated 4S RNA produced no clearly localized labeled sites over the exposure periods studied.
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1 July 1974
Article|
July 01 1974
MULTIPLE GENE SITES FOR 5S AND 18 + 28S RNA ON CHROMOSOMES OF GLYPTOTENDIPES BARBIPES (STAEGER)
Wu-Nan Wen,
Wu-Nan Wen
From the Biology Department, University of Oregon, Eugene, Oregon 97403
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Pedro E. León,
Pedro E. León
From the Biology Department, University of Oregon, Eugene, Oregon 97403
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Donald R. Hague
Donald R. Hague
From the Biology Department, University of Oregon, Eugene, Oregon 97403
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Wu-Nan Wen
From the Biology Department, University of Oregon, Eugene, Oregon 97403
Pedro E. León
From the Biology Department, University of Oregon, Eugene, Oregon 97403
Donald R. Hague
From the Biology Department, University of Oregon, Eugene, Oregon 97403
Received:
November 08 1973
Revision Received:
March 14 1974
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1974 by The Rockefeller University Press
1974
J Cell Biol (1974) 62 (1): 132–144.
Article history
Received:
November 08 1973
Revision Received:
March 14 1974
Citation
Wu-Nan Wen, Pedro E. León, Donald R. Hague; MULTIPLE GENE SITES FOR 5S AND 18 + 28S RNA ON CHROMOSOMES OF GLYPTOTENDIPES BARBIPES (STAEGER) . J Cell Biol 1 July 1974; 62 (1): 132–144. doi: https://doi.org/10.1083/jcb.62.1.132
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