The series introduced by this paper reports the results of a detailed analysis of the microsomal fraction from rat liver by density gradient centrifugation. The biochemical methods used throughout this work for the determination of monoamine oxidase, NADH cytochrome c reductase, NADPH cytochrome c reductase, cytochrome oxidase, catalase, aminopyrine demethylase, cytochromes b5 and P 450, glucuronyltransferase, galactosyltransferase, esterase, alkaline and acid phosphatases, 5'-nucleotidase, glucose 6-phosphatase, alkaline phosphodiesterase I, N-acetyl-ß-glucosaminidase, ß-glucuronidase, nucleoside diphosphatase, aldolase, fumarase, glutamine synthetase, protein, phospholipid, cholesterol, and RNA are described and justified when necessary.
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1 April 1974
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April 01 1974
ANALYTICAL STUDY OF MICROSOMES AND ISOLATED SUBCELLULAR MEMBRANES FROM RAT LIVER : I. Biochemical Methods
Henri Beaufay,
Henri Beaufay
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Alain Amar-Costesec,
Alain Amar-Costesec
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Ernest Feytmans,
Ernest Feytmans
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Denise Thinès-Sempoux,
Denise Thinès-Sempoux
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Maurice Wibo,
Maurice Wibo
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Mariette Robbi,
Mariette Robbi
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Jacques Berthet
Jacques Berthet
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
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Henri Beaufay
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Alain Amar-Costesec
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Ernest Feytmans
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Denise Thinès-Sempoux
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Maurice Wibo
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Mariette Robbi
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Jacques Berthet
From the Laboratoire de Chimie Physiologique, Université de Louvain, Louvain, Belgium
Received:
August 08 1973
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1974 by The Rockefeller University Press
1974
J Cell Biol (1974) 61 (1): 188–200.
Article history
Received:
August 08 1973
Citation
Henri Beaufay, Alain Amar-Costesec, Ernest Feytmans, Denise Thinès-Sempoux, Maurice Wibo, Mariette Robbi, Jacques Berthet; ANALYTICAL STUDY OF MICROSOMES AND ISOLATED SUBCELLULAR MEMBRANES FROM RAT LIVER : I. Biochemical Methods . J Cell Biol 1 April 1974; 61 (1): 188–200. doi: https://doi.org/10.1083/jcb.61.1.188
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