Mitosis in Fusarium oxysporum Schlect. was studied by light and electron microscopy. The average times required for the stages of mitosis, as determined from measurements made on living nuclei, were as follows: prophase, 70 sec; metaphase, 120 sec; anaphase, 13 sec; and telophase, 125 sec, for a total of 5.5 min. New postfixation procedures were developed specifically to preserve the fine-structure of the mitotic apparatus. Electron microscopy of mitotic nuclei revealed a fibrillo-granular, extranuclear Spindle Pole Body (SPB) at each pole of the intranuclear, microtubular spindles. Metaphase chromosomes were attached to spindle microtubules via kinetochores, which were found near the spindle poles at telophase. The still-intact, original nuclear envelope constricted around the incipient daughter nuclei during telophase.
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1 November 1972
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November 01 1972
ULTRASTRUCTURE AND TIME COURSE OF MITOSIS IN THE FUNGUS FUSARIUM OXYSPORUM
James R. Aist,
James R. Aist
From the Department of Plant Pathology, The University of Wisconsin, Madison, Wisconsin 53706
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P. H. Williams
P. H. Williams
From the Department of Plant Pathology, The University of Wisconsin, Madison, Wisconsin 53706
Search for other works by this author on:
James R. Aist
From the Department of Plant Pathology, The University of Wisconsin, Madison, Wisconsin 53706
P. H. Williams
From the Department of Plant Pathology, The University of Wisconsin, Madison, Wisconsin 53706
Received:
April 03 1972
Revision Received:
July 03 1972
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1972 by The Rockefeller University Press
1972
J Cell Biol (1972) 55 (2): 368–389.
Article history
Received:
April 03 1972
Revision Received:
July 03 1972
Citation
James R. Aist, P. H. Williams; ULTRASTRUCTURE AND TIME COURSE OF MITOSIS IN THE FUNGUS FUSARIUM OXYSPORUM . J Cell Biol 1 November 1972; 55 (2): 368–389. doi: https://doi.org/10.1083/jcb.55.2.368
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