RNA METABOLISM IN HELA CELLS AT REDUCED TEMPERATURE II. Steps in the Processing of Transfer RNA

Stevens , Ronald H.; Amos , Harold

doi:10.1083/jcb.54.1.1

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Article| July 01 1972

RNA METABOLISM IN HELA CELLS AT REDUCED TEMPERATURE : II. Steps in the Processing of Transfer RNA

Ronald H. Stevens,

Ronald H. Stevens

From the Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

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Harold Amos

From the Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

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Author and Article Information

Ronald H. Stevens

From the Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

Harold Amos

From the Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115

Dr. Stevens's present address is the National Institute for Medical Research, London, England.

Received: September 27 1971

Revision Received: March 27 1972

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1972

J Cell Biol (1972) 54 (1): 1–7.

https://doi.org/10.1083/jcb.54.1.1

Incubation of HeLa cells at 24°C results in the modification of the processing of pre-tRNA to tRNA. Both methylation and size reduction were shown to take place in vitro when purified pre-tRNA was subjected to processing in a cytoplasmic extract of HeLa cells The migration of pre-tRNA from the nucleus to the cytoplasm was not significantly altered at 24°C

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