THE MYOFILAMENT LATTICE: STUDIES ON ISOLATED FIBERS II. The Effects of Osmotic Strength, Ionic Concentration, and pH upon the Unit-Cell Volume

April , Ernest W.; Brandt , Philip W.; Elliott , Gerald F.

doi:10.1083/jcb.53.1.53

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Article| April 01 1972

THE MYOFILAMENT LATTICE: STUDIES ON ISOLATED FIBERS : II. The Effects of Osmotic Strength, Ionic Concentration, and pH upon the Unit-Cell Volume

Ernest W. April,

Ernest W. April

From the Department of Anatomy, College of Physicians and Surgeons of Columbia University, New York 10032; the Department of Chemistry, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213; and the Marine Biological Laboratory, Woods Hole, Massachusetts 02543.

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Philip W. Brandt,

Philip W. Brandt

From the Department of Anatomy, College of Physicians and Surgeons of Columbia University, New York 10032; the Department of Chemistry, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213; and the Marine Biological Laboratory, Woods Hole, Massachusetts 02543.

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Gerald F. Elliott

From the Department of Anatomy, College of Physicians and Surgeons of Columbia University, New York 10032; the Department of Chemistry, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213; and the Marine Biological Laboratory, Woods Hole, Massachusetts 02543.

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Author and Article Information

Ernest W. April

From the Department of Anatomy, College of Physicians and Surgeons of Columbia University, New York 10032; the Department of Chemistry, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213; and the Marine Biological Laboratory, Woods Hole, Massachusetts 02543.

Philip W. Brandt

From the Department of Anatomy, College of Physicians and Surgeons of Columbia University, New York 10032; the Department of Chemistry, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213; and the Marine Biological Laboratory, Woods Hole, Massachusetts 02543.

Gerald F. Elliott

From the Department of Anatomy, College of Physicians and Surgeons of Columbia University, New York 10032; the Department of Chemistry, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213; and the Marine Biological Laboratory, Woods Hole, Massachusetts 02543.

Dr. Elliott's present address is the Department of Physics, The Open University, Bletchley, Buckinghamshire, England.

Received: July 14 1971

Revision Received: December 09 1971

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1972

J Cell Biol (1972) 53 (1): 53–65.

https://doi.org/10.1083/jcb.53.1.53

The effects of osmotic concentration, ionic strength, and pH on the myofilament lattice spacing of intact and skinned single fibers from the walking leg of crayfish (Orconectes) were determined by electron microscopy and low-angle X-ray diffraction. Sarcomere lengths were determined by light diffraction. It is demonstrated that the interfilament spacing in the intact fiber is a function of the volume of the fiber. It is also shown that the interfilament spacing of the skinned (but not of the intact) fiber is affected in a predictable manner by ionic strength and pH insofar as these parameters affect the electrostatic repulsive forces between the filaments. From these combined observations it is demonstrated that the unit-cell volume of the in vivo myofilament lattice behaves in a manner similar to that described for liquid-crystalline solutions.

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THE MYOFILAMENT LATTICE: STUDIES ON ISOLATED FIBERS : II. The Effects of Osmotic Strength, Ionic Concentration, and pH upon the Unit-Cell Volume

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