A rapid and convenient method has been utilized to investigate glucose oxidation during growth of chick embryo heart cells in tissue culture. Primary isolates of chick embryo heart cells showed exponential growth when plated at low densities and exhibited density-inhibited growth as cultures became confluent. The density-dependent growth inhibition of chick embryo heart cells is associated with a marked decrease in the specific activity of glucose oxidation to CO2. This decrease in glucose oxidation was observed as density increased as either a function of time in culture or as related to initial plating density. The decrease in 14CO2 production associated with density-dependent inhibition of growth is due to a marked decrease in activity of the pentose phosphate pathway.
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1 February 1972
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February 01 1972
CHANGES IN GLUCOSE OXIDATION DURING GROWTH OF EMBRYONIC HEART CELLS IN CULTURE
Joseph B. Warshaw,
Joseph B. Warshaw
From the Children' Service, Massachusetts General Hospital, Shriners Burns Institute, and the Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02114
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Miriam D. Rosenthal
Miriam D. Rosenthal
From the Children' Service, Massachusetts General Hospital, Shriners Burns Institute, and the Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02114
Search for other works by this author on:
Joseph B. Warshaw
From the Children' Service, Massachusetts General Hospital, Shriners Burns Institute, and the Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02114
Miriam D. Rosenthal
From the Children' Service, Massachusetts General Hospital, Shriners Burns Institute, and the Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02114
Received:
July 23 1971
Revision Received:
September 30 1971
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1972 by The Rockefeller University Press
1972
J Cell Biol (1972) 52 (2): 283–291.
Article history
Received:
July 23 1971
Revision Received:
September 30 1971
Citation
Joseph B. Warshaw, Miriam D. Rosenthal; CHANGES IN GLUCOSE OXIDATION DURING GROWTH OF EMBRYONIC HEART CELLS IN CULTURE . J Cell Biol 1 February 1972; 52 (2): 283–291. doi: https://doi.org/10.1083/jcb.52.2.283
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