DEXTRANS AND GLYCOGENS AS PARTICULATE TRACERS FOR STUDYING CAPILLARY PERMEABILITY

Simionescu , Nicolae; Palade , George E.

doi:10.1083/jcb.50.3.616

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Article| September 01 1971

DEXTRANS AND GLYCOGENS AS PARTICULATE TRACERS FOR STUDYING CAPILLARY PERMEABILITY

Nicolae Simionescu,

Nicolae Simionescu

From The Rockefeller University, New York 10021.

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George E. Palade

From The Rockefeller University, New York 10021.

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Author and Article Information

Nicolae Simionescu

From The Rockefeller University, New York 10021.

George E. Palade

From The Rockefeller University, New York 10021.

Dr. Simionescu's permanent address is Institute of Endocrinology, Bucharest, Romania

Received: November 20 1970

Revision Received: January 04 1971

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1971

J Cell Biol (1971) 50 (3): 616–624.

https://doi.org/10.1083/jcb.50.3.616

Commercially available glycogens and dextrans can be used as biological particulate tracers in work on capillary permeability. These polysaccharides are well tolerated in intravenous injection and induce no vascular leakage when applied topically (cremaster test) in mice and in Wistar-Furth rats. The particles stain adequately with lead after aldehyde-OsO₄ fixation in phosphate buffer and provide a relatively wide set of probes (∼45 A-300 A) for work on the large and small pore systems.

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