ULTRASTRUCTURAL LOCALIZATION OF CALCITONIN IN THE PARAFOLLICULAR CELLS OF PIG THYROID GLAND WITH CYTOCHROME c-LABELED ANTIBODY FRAGMENTS

De Grandi , P. B.; Kraehenbuhl , J. P.; Campiche , M. A.

doi:10.1083/jcb.50.2.446

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Article| August 01 1971

ULTRASTRUCTURAL LOCALIZATION OF CALCITONIN IN THE PARAFOLLICULAR CELLS OF PIG THYROID GLAND WITH CYTOCHROME c-LABELED ANTIBODY FRAGMENTS

P. B. De Grandi,

P. B. De Grandi

From the Institute of Pathological Anatomy, University of Zurich, and the Institute of Pathological Anatomy, University of Lausanne, Switzerland.

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J. P. Kraehenbuhl,

J. P. Kraehenbuhl

From the Institute of Pathological Anatomy, University of Zurich, and the Institute of Pathological Anatomy, University of Lausanne, Switzerland.

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M. A. Campiche

From the Institute of Pathological Anatomy, University of Zurich, and the Institute of Pathological Anatomy, University of Lausanne, Switzerland.

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Author and Article Information

P. B. De Grandi

From the Institute of Pathological Anatomy, University of Zurich, and the Institute of Pathological Anatomy, University of Lausanne, Switzerland.

J. P. Kraehenbuhl

From the Institute of Pathological Anatomy, University of Zurich, and the Institute of Pathological Anatomy, University of Lausanne, Switzerland.

M. A. Campiche

From the Institute of Pathological Anatomy, University of Zurich, and the Institute of Pathological Anatomy, University of Lausanne, Switzerland.

Dr. De Grandi's present address is the Department of Surgery, University Hospital, Lausanne, Switzerland. Dr. Kraehenbuhl's present address is The Rockefeller University, New York 10021

Received: October 20 1970

Revision Received: March 05 1971

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1971

J Cell Biol (1971) 50 (2): 446–456.

https://doi.org/10.1083/jcb.50.2.446

Parafollicular cells in mammalian thyroid glands are thought to be responsible for the secretion of calcitonin. In this study, calcitonin was localized in pig thyroid gland by an indirect immunocytochemical technique using rabbit antiserum directed against synthetic porcine calcitonin for the first step, and sheep Fab fragments prepared against rabbit Fab and coupled to cytochrome c for the second step. The antigenic determinants of calcitonin were present only in the parafollicular cells, whose secretory granules were heavily labeled. Labeling of the cytoplasmic matrix is thought to indicate a possible leakage of the polypeptide from the granules. A striking observation was the complete absence of labeling in the cisternae of the rough-surfaced endoplasmic reticulum and of the Golgi apparatus. It is concluded that the secretory granules of parafollicular cells contain calcitonin; the mechanism of synthesis of this peptide is not clearly understood.

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ULTRASTRUCTURAL LOCALIZATION OF CALCITONIN IN THE PARAFOLLICULAR CELLS OF PIG THYROID GLAND WITH CYTOCHROME c-LABELED ANTIBODY FRAGMENTS

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