The dependence of nucleolar reformation on RNA synthesis that resumes in late anaphase or early telophase has been investigated in synchronously dividing Amoeba proteus. RNA synthesis was completely inhibited throughout all stages of mitosis and the early hours of interphase with high concentrations of actinomycin D. In such cells, nucleolus-like bodies that bind azure B and pyronin were apparent in the reformed nuclei. The bodies appear as dense, fibrous masses with loosely associated, finely fibrillar material. There are no characteristic granular regions in the reformed structures. It is suggested that the bodies probably represent mainly nucleolar protein and residual RNA which can bring about the reorganization of nucleoli in the absence of postmitotic RNA synthesis.
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1 March 1971
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March 01 1971
REFORMATION OF NUCLEOLUS-LIKE BODIES IN THE ABSENCE OF POSTMITOTIC RNA SYNTHESIS
A. R. Stevens,
A. R. Stevens
From the Medical Research Service, Veterans Administration Hospital, and The Department of Anatomical Sciences, University of Florida College of Medicine, Gainesville, Florida 32601; and the Department of Molecular, Cellular and Developmental Biology, the University of Colorado, Boulder, Colorado 80302
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D. M. Prescott
D. M. Prescott
From the Medical Research Service, Veterans Administration Hospital, and The Department of Anatomical Sciences, University of Florida College of Medicine, Gainesville, Florida 32601; and the Department of Molecular, Cellular and Developmental Biology, the University of Colorado, Boulder, Colorado 80302
Search for other works by this author on:
A. R. Stevens
From the Medical Research Service, Veterans Administration Hospital, and The Department of Anatomical Sciences, University of Florida College of Medicine, Gainesville, Florida 32601; and the Department of Molecular, Cellular and Developmental Biology, the University of Colorado, Boulder, Colorado 80302
D. M. Prescott
From the Medical Research Service, Veterans Administration Hospital, and The Department of Anatomical Sciences, University of Florida College of Medicine, Gainesville, Florida 32601; and the Department of Molecular, Cellular and Developmental Biology, the University of Colorado, Boulder, Colorado 80302
Received:
May 12 1970
Revision Received:
October 13 1970
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1971 by The Rockefeller University Press
1971
J Cell Biol (1971) 48 (3): 443–454.
Article history
Received:
May 12 1970
Revision Received:
October 13 1970
Citation
A. R. Stevens, D. M. Prescott; REFORMATION OF NUCLEOLUS-LIKE BODIES IN THE ABSENCE OF POSTMITOTIC RNA SYNTHESIS . J Cell Biol 1 March 1971; 48 (3): 443–454. doi: https://doi.org/10.1083/jcb.48.3.443
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