MASS PREPARATION OF NUCLEI FROM THE LARVAL SALIVARY GLANDS OF DROSOPHILA HYDEI

Boyd , James B.; Berendes , Hans D.; Boyd , Hudson

doi:10.1083/jcb.38.2.369

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Article| August 01 1968

MASS PREPARATION OF NUCLEI FROM THE LARVAL SALIVARY GLANDS OF DROSOPHILA HYDEI

James B. Boyd,

James B. Boyd

From the Max-Planck-Institut für Biologic, Tübingen, Germany

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Hans D. Berendes,

Hans D. Berendes

From the Max-Planck-Institut für Biologic, Tübingen, Germany

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Hudson Boyd

From the Max-Planck-Institut für Biologic, Tübingen, Germany

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Author and Article Information

James B. Boyd

From the Max-Planck-Institut für Biologic, Tübingen, Germany

Hans D. Berendes

From the Max-Planck-Institut für Biologic, Tübingen, Germany

Hudson Boyd

From the Max-Planck-Institut für Biologic, Tübingen, Germany

Received: February 26 1968

Online ISSN: 1540-8140

Print ISSN: 0021-9525

1968

J Cell Biol (1968) 38 (2): 369–376.

https://doi.org/10.1083/jcb.38.2.369

A method has been developed for isolating gram quantities of salivary glands from late third instar larvae of Drosophila hydei. The isolated glands have a normal appearance and incorporate RNA and DNA precursors normally. Nuclei can be isolated from these glands in 90% yield with the use of detergents. These nuclei contain morphologically normal giant polytene chromosomes.

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