Thick myosin filaments, in addition to actin filaments, were found in sections of glycerinated chicken gizzard smooth muscle when fixed at a pH below 6.6. The thick filaments were often grouped into bundles and run in the longitudinal axis of the smooth muscle cell. Each thick filament was surrounded by a number of thin filaments, giving the filament arrangement a rosette appearance in cross-section. The exact ratio of thick filaments to thin filaments could not be determined since most arrays were not so regular as those commonly found in striated muscle. Some rosettes had seven or eight thin filaments surrounding a single thick filament. Homogenates of smooth muscle of chicken gizzard also showed both thick and thin filaments when the isolation was carried out at a pH below 6.6, but only thin filaments were found at pH 7.4. No Z or M lines were observed in chicken gizzard muscle containing both thick and thin filaments. The lack of these organizing structures may allow smooth muscle myosin to disaggregate readily at pH 7.4.
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1 April 1968
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April 01 1968
LOCALIZATION OF MYOSIN FILAMENTS IN SMOOTH MUSCLE
Robert E. Kelly,
Robert E. Kelly
From the Mellon Institute of Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213
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Robert V. Rice
Robert V. Rice
From the Mellon Institute of Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213
Search for other works by this author on:
Robert E. Kelly
From the Mellon Institute of Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213
Robert V. Rice
From the Mellon Institute of Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213
Received:
August 09 1967
Revision Received:
October 24 1967
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1968
J Cell Biol (1968) 37 (1): 105–116.
Article history
Received:
August 09 1967
Revision Received:
October 24 1967
Citation
Robert E. Kelly, Robert V. Rice; LOCALIZATION OF MYOSIN FILAMENTS IN SMOOTH MUSCLE . J Cell Biol 1 April 1968; 37 (1): 105–116. doi: https://doi.org/10.1083/jcb.37.1.105
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