The sites of lead phosphate precipitation in mouse bladder smooth muscle incubated with adenosine triphosphate and lead nitrate were studied by electron microscopy. The media constituents and incubating conditions were independently varied so that we could determine optimal conditions for histochemical demonstration of ATPase activity in agranular endoplasmic reticulum. Specimens of glutaraldehyde-fixed bladder muscle, frozen, cut into 10–40-µ sections, and incubated for 1 hr at 25°C in medium containing 0.025 M ATP, 0.0025 M lead nitrate, 0.05 M magnesium chloride, and 0.09 M sodium acetate buffer at pH 6.2, exhibited microcrystalline deposits in agranular endoplasmic reticulum and pinocytotic vesicles. Lead salt deposition was also noted in terminal cisternae of sarcoplasmic reticulum in skeletal muscle similarly treated, suggesting that the organelle systems in the two types of muscle cells subserve a common function.
Skip Nav Destination
Article navigation
1 September 1967
Article|
September 01 1967
LOCALIZATION OF PRODUCTS OF ATP HYDROLYSIS IN MAMMALIAN SMOOTH MUSCLE CELLS
Bernard P. Lane
Bernard P. Lane
From the Departments of Pathology and Anatomy, New York University School of Medicine, New York, 10016
Search for other works by this author on:
Bernard P. Lane
From the Departments of Pathology and Anatomy, New York University School of Medicine, New York, 10016
Received:
September 06 1966
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1967 by The Rockefeller University Press
1967
J Cell Biol (1967) 34 (3): 713–720.
Article history
Received:
September 06 1966
Citation
Bernard P. Lane; LOCALIZATION OF PRODUCTS OF ATP HYDROLYSIS IN MAMMALIAN SMOOTH MUSCLE CELLS . J Cell Biol 1 September 1967; 34 (3): 713–720. doi: https://doi.org/10.1083/jcb.34.3.713
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
Advertisement