The generation cycle of germinative cells (external matrix cells) in the external granular layer of the cerebellar cortex of the 10-to 11-day-old mouse was studied by radioautography following repeated injections of H3-thymidine. The generation time is 19 hr, presynthetic time 8.5 hr, DNA-synthetic time 8 hr, postsynthetic time 2 hr, and mitotic time 0.5 hr. These proliferating cells occupy the outer half of the external granular layer and make up the external matrix layer. Neuroblasts are differentiated from the external matrix cell, migrate out from the layer and accumulate in the inner half of the external granular layer to form the external mantle layer. The transit time of the neuroblasts in the external mantle layer is 28 hr. Thereafter, they migrate farther into the molecular layer and the internal granular layer. By means of long-term cumulative labeling, the rate of daily production of neuroblasts from the external matrix cell is studied in quantitative terms. It becomes clear that the entire population of the inner granule neurons arises postnatally in the external granular layer between 1 and 18 days of age and that 95% of them is produced between postnatal days 4 and 15. Finally, the fate of the cells in the external granular layer at its terminal stage was studied by marking the cells with H3-thymidine during 15–16 days of life and following their subsequent migration and developmental changes up to 21 days of life. Comparison of radioautographs taken before and after the migration disclosed that the external matrix cells give rise to a small number of neuroglia cells. This finding revealed their multipotential nature.
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1 February 1967
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February 01 1967
QUANTITATIVE ANALYSIS OF CELL PROLIFERATION AND DIFFERENTIATION IN THE CORTEX OF THE POSTNATAL MOUSE CEREBELLUM
Setsuya Fujita
Setsuya Fujita
From the Department of Biological Sciences, Purdue University, Lafayette, Indiana
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Setsuya Fujita
From the Department of Biological Sciences, Purdue University, Lafayette, Indiana
Received:
July 21 1966
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1967 by The Rockefeller University Press
1967
J Cell Biol (1967) 32 (2): 277–287.
Article history
Received:
July 21 1966
Citation
Setsuya Fujita; QUANTITATIVE ANALYSIS OF CELL PROLIFERATION AND DIFFERENTIATION IN THE CORTEX OF THE POSTNATAL MOUSE CEREBELLUM . J Cell Biol 1 February 1967; 32 (2): 277–287. doi: https://doi.org/10.1083/jcb.32.2.277
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