Meiotic chromosomes were isolated from male Oncopeltus fasciatus by dissecting the testes under insect Ringer's solution and spreading the living cells on the Langmuir trough. After being dried by the critical point method, preparations were examined under the electron microscope. Chromosomes at all stages of prophase prove to be multistranded. A significant increase in the number of parallel 250 A fibers in the chromosomes occurs between zygotene and diakinesis. Parallel folding, rather than true multistrandedness, is interpreted as the mechanism responsible for this observed increase in multistrandedness. It has not been possible to determine whether the multistrandedness observed at leptotene represents true multistrandedness or is the result of parallel folding. Apparent multistrandedness is lost at metaphase when the 250 A fibers of the chromosomes become coiled more tightly. In preparations isolated by these methods, no structures other than the 250 A chromosome fibers are visible in the chromomeres, which appear as regionally coiled or folded areas of the fibers along the arm of the chromosome.
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1 October 1966
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October 01 1966
THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS
Stephen L. Wolfe,
Stephen L. Wolfe
From the Departments of Zoology and Genetics, University of California, Davis
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Godfrey M. Hewitt
Godfrey M. Hewitt
From the Departments of Zoology and Genetics, University of California, Davis
Search for other works by this author on:
Stephen L. Wolfe
From the Departments of Zoology and Genetics, University of California, Davis
Godfrey M. Hewitt
From the Departments of Zoology and Genetics, University of California, Davis
Received:
April 01 1966
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1966 by The Rockefeller University Press
1966
J Cell Biol (1966) 31 (1): 31–42.
Article history
Received:
April 01 1966
Citation
Stephen L. Wolfe, Godfrey M. Hewitt; THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS . J Cell Biol 1 October 1966; 31 (1): 31–42. doi: https://doi.org/10.1083/jcb.31.1.31
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