Increased alkaline phosphatase activity is induced in certain epithelial cell cultures by hormones with adrenal glucocorticoid activity or their analogues such as prednisolone (ΔI-hydrocortisone). Enzyme induction occurs in two distinct phases. During the first 12 hr after the addition of prednisolone, there is a small increase in alkaline phosphatase levels. After 15 to 24 hr, the enzyme activity shows a sudden, marked linear rise, reaching a maximum at 60 to 80 hr. Puromycin blocks enzyme induction immediately, even when added during the period of rapid increase of enzyme. Actinomycin D blocks induction when added no later than 8 hr after the addition of prednisolone. On the other hand, Actinomycin D added during the phase of rapid enzyme induction has no effect for at least 12 hr. These findings suggest that de novo protein synthesis is involved in prednisolone induction of alkaline phosphatase and that the RNA messenger for this enzyme is relatively stable.
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1 April 1966
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April 01 1966
STUDIES ON THE MECHANISM OF HORMONAL INDUCTION OF ALKALINE PHOSPHATASE IN HUMAN CELL CULTURES : I. Effects of Puromycin and Actinomycin D
Martin J. Griffin,
Martin J. Griffin
From the Department of Medicine, New York University School of Medicine, New York, and The Summer Research Institute of the Will Rogers Hospital, Saranac Lake, New York.
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Rody P. Cox
Rody P. Cox
From the Department of Medicine, New York University School of Medicine, New York, and The Summer Research Institute of the Will Rogers Hospital, Saranac Lake, New York.
Search for other works by this author on:
Martin J. Griffin
From the Department of Medicine, New York University School of Medicine, New York, and The Summer Research Institute of the Will Rogers Hospital, Saranac Lake, New York.
Rody P. Cox
From the Department of Medicine, New York University School of Medicine, New York, and The Summer Research Institute of the Will Rogers Hospital, Saranac Lake, New York.
Dr. Griffin's present address is the Department of Cancer Research, University of Oklahoma School of Medicine, Oklahoma City
Received:
July 19 1965
Online ISSN: 1540-8140
Print ISSN: 0021-9525
1966
J Cell Biol (1966) 29 (1): 1–9.
Article history
Received:
July 19 1965
Citation
Martin J. Griffin, Rody P. Cox; STUDIES ON THE MECHANISM OF HORMONAL INDUCTION OF ALKALINE PHOSPHATASE IN HUMAN CELL CULTURES : I. Effects of Puromycin and Actinomycin D . J Cell Biol 1 April 1966; 29 (1): 1–9. doi: https://doi.org/10.1083/jcb.29.1.1
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