Studies comparing the state of hydration and dehydration of rat liver mitochondria to their content of ATP, Ca, and fatty acid, along with the rate of ATP hydrolysis, as well as microscopic appearance of mitochondria, have led to the following generalizations: 1. The competition between cationic translocations and water translocation for the available chemical energy (ATP) determines under many circumstances the water content of mitochondria. 2. Swelling of mitochondria by electron transport substrates is an example of the activation of the cationic translocations at the expense of water translocation. 3. Electron micrographic studies are interpreted to indicate that EDTA alone can cause condensation and dehydration of the mitochondrial matrix. However, both EDTA and substrate are necessary to remove appreciable quantities of water from mitochondrial intramembranous spaces. 4. Since the data in the accompanying report indicated that EDTA, in the absence of energy, decreased the permeability of mitochondrial membranes, it appears likely that ballooning of intramembranous spaces, following addition of EDTA, represents trapping of water between two semipermeable membranes following dehydration of mitochondrial matrix.
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1 October 1964
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October 01 1964
ROLE OF EDTA AND METALS IN MITOCHONDRIAL CONTRACTION
William S. Lynn, Jr.,
William S. Lynn, Jr.
From the Departments of Biochemistry and Medicine, Duke University Medical Center, Durham, North Carolina
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Sydney Fortney,
Sydney Fortney
From the Departments of Biochemistry and Medicine, Duke University Medical Center, Durham, North Carolina
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Rose H. Brown
Rose H. Brown
From the Departments of Biochemistry and Medicine, Duke University Medical Center, Durham, North Carolina
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William S. Lynn, Jr.
From the Departments of Biochemistry and Medicine, Duke University Medical Center, Durham, North Carolina
Sydney Fortney
From the Departments of Biochemistry and Medicine, Duke University Medical Center, Durham, North Carolina
Rose H. Brown
From the Departments of Biochemistry and Medicine, Duke University Medical Center, Durham, North Carolina
Received:
October 24 1963
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Copyright © 1964 by The Rockefeller Institute Press
1964
J Cell Biol (1964) 23 (1): 9–19.
Article history
Received:
October 24 1963
Citation
William S. Lynn, Sydney Fortney, Rose H. Brown; ROLE OF EDTA AND METALS IN MITOCHONDRIAL CONTRACTION . J Cell Biol 1 October 1964; 23 (1): 9–19. doi: https://doi.org/10.1083/jcb.23.1.9
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