The process of folding the flat neuroectoderm into an elongated neural tube depends on tissue fluidity, a property that allows epithelial deformation while preserving tissue integrity. Neural tube folding also requires the planar cell polarity (PCP) pathway. Here, we report that Prickle2 (Pk2), a core PCP component, increases tissue fluidity by promoting the remodeling of apical junctions (AJs) in Xenopus embryos. This Pk2 activity is mediated by the unique evolutionarily conserved Ser/Thr-rich region (STR) in the carboxyterminal half of the protein. Mechanistically, the effects of Pk2 require Rac1 and are accompanied by increased dynamics of C-cadherin and tricellular junctions, the hotspots of AJ remodeling. Notably, Pk2 depletion leads to the accumulation of mediolaterally oriented cells in the neuroectoderm, whereas the overexpression of Pk2 or Pk1 containing the Pk2-derived STR promotes cell elongation along the anteroposterior axis. We propose that Pk2-dependent regulation of tissue fluidity contributes to anteroposterior tissue elongation in response to extrinsic cues.
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February 14 2025
Prickle2 regulates apical junction remodeling and tissue fluidity during vertebrate neurulation
Miho Matsuda
,
(Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing - original draft, Writing - review & editing)
1Department of Cell, Developmental and Regenerative Biology,
Icahn School of Medicine at Mount Sinai
, New York, NY, USA
Miho Matsuda: [email protected]
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Sergei Y. Sokol
(Conceptualization, Funding acquisition, Methodology, Project administration, Resources, Supervision, Writing - original draft, Writing - review & editing)
1Department of Cell, Developmental and Regenerative Biology,
Icahn School of Medicine at Mount Sinai
, New York, NY, USA
Correspondence to Sergei Y. Sokol: [email protected]
Search for other works by this author on:
Miho Matsuda
Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing - original draft, Writing - review & editing
1Department of Cell, Developmental and Regenerative Biology,
Icahn School of Medicine at Mount Sinai
, New York, NY, USA
Correspondence to Sergei Y. Sokol: [email protected]
Miho Matsuda: [email protected]
Disclosures: The authors declare no competing interests exist.
Received:
July 02 2024
Revision Received:
December 29 2024
Accepted:
January 21 2025
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Funding
Funder(s):
National Institutes of Health
- Award Id(s): R35GM122492
© 2025 Matsuda and Sokol
2025
Matsuda and Sokol
This article is distributed under the terms as described at https://rupress.org/pages/terms102024/.
J Cell Biol (2025) 224 (4): e202407025.
Article history
Received:
July 02 2024
Revision Received:
December 29 2024
Accepted:
January 21 2025
Citation
Miho Matsuda, Sergei Y. Sokol; Prickle2 regulates apical junction remodeling and tissue fluidity during vertebrate neurulation. J Cell Biol 3 April 2025; 224 (4): e202407025. doi: https://doi.org/10.1083/jcb.202407025
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