Here, we report the generation of a transgenic Lifeact–EGFP quail line for the investigation of actin organization and dynamics during morphogenesis in vivo. This transgenic avian line allows for the high-resolution visualization of actin structures within the living embryo, from the subcellular filaments that guide cell shape to the supracellular assemblies that coordinate movements across tissues. The unique suitability of avian embryos to live imaging facilitates the investigation of previously intractable processes during embryogenesis. Using high-resolution live imaging approaches, we present the dynamic behaviors and morphologies of cellular protrusions in different tissue contexts. Furthermore, through the integration of live imaging with computational segmentation, we visualize cells undergoing apical constriction and large-scale actin structures such as multicellular rosettes within the neuroepithelium. These findings not only enhance our understanding of tissue morphogenesis but also demonstrate the utility of the Lifeact–EGFP transgenic quail as a new model system for live in vivo investigations of the actin cytoskeleton.
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2 September 2024
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June 24 2024
A Lifeact-EGFP quail for studying actin dynamics in vivo
Yanina D. Alvarez
,
(Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing - review & editing)
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
Yanina D. Alvarez: [email protected]
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Marise van der Spuy
,
Marise van der Spuy
(Formal analysis, Investigation, Methodology, Writing - original draft)
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
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Jian Xiong Wang
,
Jian Xiong Wang
(Formal analysis, Investigation, Methodology, Writing - review & editing)
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
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Ivar Noordstra
,
Ivar Noordstra
(Formal analysis, Investigation, Writing - review & editing)
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
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Siew Zhuan Tan
,
Siew Zhuan Tan
(Investigation, Methodology, Writing - review & editing)
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
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Murron Carroll
,
Murron Carroll
(Formal analysis, Writing - review & editing)
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
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Alpha S. Yap
,
Alpha S. Yap
(Investigation)
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
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Olivier Serralbo
,
Olivier Serralbo
(Resources)
2
Commonwealth Scientific and Industrial Research (CSIRO) Health and Biosecurity
, Geelong, Australia
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Melanie D. White
(Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Validation, Visualization, Writing - original draft, Writing - review & editing)
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
3School of Biomedical Sciences, The University of Queensland, Brisbane, Australia
Correspondence to Melanie D. White: [email protected]
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Yanina D. Alvarez
Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing - review & editing
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
Marise van der Spuy
Formal analysis, Investigation, Methodology, Writing - original draft
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
Jian Xiong Wang
Formal analysis, Investigation, Methodology, Writing - review & editing
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
Ivar Noordstra
Formal analysis, Investigation, Writing - review & editing
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
Siew Zhuan Tan
Investigation, Methodology, Writing - review & editing
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
Murron Carroll
Formal analysis, Writing - review & editing
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
Alpha S. Yap
Investigation
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
Olivier Serralbo
Resources
2
Commonwealth Scientific and Industrial Research (CSIRO) Health and Biosecurity
, Geelong, Australia
Melanie D. White
Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Project administration, Supervision, Validation, Visualization, Writing - original draft, Writing - review & editing
1Institute for Molecular Bioscience,
The University of Queensland
, Brisbane, Australia
3School of Biomedical Sciences, The University of Queensland, Brisbane, Australia
Correspondence to Melanie D. White: [email protected]
Yanina D. Alvarez: [email protected]
Disclosures: The authors declare no competing interests exist.
Received:
April 12 2024
Revision Received:
May 15 2024
Accepted:
June 03 2024
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Funder(s):
Australian Cancer Research Foundation
Funder(s):
Australian Research Council
- Award Id(s): FT200100899,DP220101878,DP19010287,190102230
Funder(s):
National Health and Medical Research Council of Australia
- Award Id(s): 2013027,GNT1163462,201070,GNT1136592
Funder(s):
European Molecular Biology Organization
- Award Id(s): ALTF 251-2018
© 2024 Alvarez et al.
2024
Alvarez et al.
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
J Cell Biol (2024) 223 (9): e202404066.
Article history
Received:
April 12 2024
Revision Received:
May 15 2024
Accepted:
June 03 2024
Citation
Yanina D. Alvarez, Marise van der Spuy, Jian Xiong Wang, Ivar Noordstra, Siew Zhuan Tan, Murron Carroll, Alpha S. Yap, Olivier Serralbo, Melanie D. White; A Lifeact-EGFP quail for studying actin dynamics in vivo. J Cell Biol 2 September 2024; 223 (9): e202404066. doi: https://doi.org/10.1083/jcb.202404066
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