Two protocadherins, Dachsous and Fat, regulate organ growth in Drosophila via the Hippo pathway. Dachsous and Fat bind heterotypically to regulate the abundance and subcellular localization of a “core complex” consisting of Dachs, Dlish, and Approximated. This complex localizes to the junctional cortex where it represses Warts. Dachsous is believed to promote growth by recruiting and stabilizing this complex, while Fat represses growth by promoting its degradation. Here, we examine the functional relationships between the intracellular domains of Dachsous and Fat and the core complex. While Dachsous promotes the accumulation of core complex proteins in puncta, it is not required for their assembly. Indeed, the core complex accumulates maximally in the absence of both Dachsous and Fat. Furthermore, Dachsous represses growth in the absence of Fat by removing the core complex from the junctional cortex. Fat similarly recruits core complex components but promotes their degradation. Our findings reveal that Dachsous and Fat coordinately constrain tissue growth by repressing the core complex.
Skip Nav Destination
Article navigation
Article|
October 07 2024
Dachsous and Fat coordinately repress the Dachs–Dlish–Approximated complex to control growth
Hitoshi Matakatsu
,
Hitoshi Matakatsu
(Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Validation, Visualization, Writing - original draft, Writing - review & editing)
1Department of Molecular Genetics and Cell Biology,
University of Chicago
, Chicago, IL, USA
Search for other works by this author on:
Richard G. Fehon
(Conceptualization, Funding acquisition, Project administration, Supervision, Validation, Writing - original draft, Writing - review & editing)
1Department of Molecular Genetics and Cell Biology,
University of Chicago
, Chicago, IL, USA
Correspondence to Richard G. Fehon: [email protected]
Search for other works by this author on:
Hitoshi Matakatsu
Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Validation, Visualization, Writing - original draft, Writing - review & editing
1Department of Molecular Genetics and Cell Biology,
University of Chicago
, Chicago, IL, USA
Correspondence to Richard G. Fehon: [email protected]
Disclosures: The authors declare no competing interests exist.
Received:
June 19 2024
Revision Received:
August 28 2024
Accepted:
September 11 2024
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Funder(s):
National Institutes of Health
- Award Id(s): GM134128
© 2024 Matakatsu and Fehon
2024
Matakatsu and Fehon
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
J Cell Biol (2024) 223 (12): e202406119.
Article history
Received:
June 19 2024
Revision Received:
August 28 2024
Accepted:
September 11 2024
Citation
Hitoshi Matakatsu, Richard G. Fehon; Dachsous and Fat coordinately repress the Dachs–Dlish–Approximated complex to control growth. J Cell Biol 2 December 2024; 223 (12): e202406119. doi: https://doi.org/10.1083/jcb.202406119
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your Institution
405
Views
Suggested Content
Email alerts
Advertisement
Advertisement