It is known that microtubule-binding proteins including the Ska1 complex and the DNA replication licensing factor, Cdt1, enable the kinetochore-localized Ndc80 complex to form robust kinetochore-microtubule attachments. However, it is not clear how the Ndc80 complex is stably coupled to dynamic spindle microtubule plus-ends. Here, we have developed a conditional auxin-inducible degron approach to reveal a function for Cdt1 in chromosome segregation and kinetochore–microtubule interactions that is separable from its role in DNA replication licensing. Further, we demonstrate that a direct interaction between Cdt1 and Ska1 is required for recruiting Cdt1 to kinetochores and spindle microtubules. Cdt1 phosphorylation by Cdk1 kinase is critical for Ska1 binding, kinetochore–microtubule attachments, and mitotic progression. Furthermore, we show that Cdt1 synergizes with Ndc80 and Ska1 for microtubule binding, including forming a diffusive, tripartite Ndc80-Cdt1-Ska1 complex that can processively track dynamic microtubule plus-ends in vitro. Taken together, our data identify the Ndc80-Cdt1-Ska1 complex as a central molecular unit that can promote processive bidirectional tip-tracking of microtubules by kinetochores.
The Ndc80-Cdt1-Ska1 complex is a central processive kinetochore–microtubule coupling unit
Disclosures: The authors declare no competing interests exist.
Shivangi Agarwal’s current affiliation is Grove Biopharma Inc., Chicago, IL, USA.
Shivani Agarwal’s current affiliation is Division of Hematology/Oncology, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA.
- Award Id(s): R01GM135391,R35GM124889
Amit Rahi, Manas Chakraborty, Shivangi Agarwal, Kristen M. Vosberg, Shivani Agarwal, Annie Y. Wang, Richard J. McKenney, Dileep Varma; The Ndc80-Cdt1-Ska1 complex is a central processive kinetochore–microtubule coupling unit. J Cell Biol 7 August 2023; 222 (8): e202208018. doi: https://doi.org/10.1083/jcb.202208018
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