Cells migrate collectively through confined environments during development and cancer metastasis. The nucleus, a stiff organelle, impedes single cells from squeezing into narrow channels within artificial environments. However, how nuclei affect collective migration into compact tissues is unknown. Here, we use border cells in the fly ovary to study nuclear dynamics in collective, confined in vivo migration. Border cells delaminate from the follicular epithelium and squeeze into tiny spaces between cells called nurse cells. The lead cell nucleus transiently deforms within the lead cell protrusion, which then widens. The nuclei of follower cells deform less. Depletion of the Drosophila B-type lamin, Lam, compromises nuclear integrity, hinders expansion of leading protrusions, and impedes border cell movement. In wildtype, cortical myosin II accumulates behind the nucleus and pushes it into the protrusion, whereas in Lam-depleted cells, myosin accumulates but does not move the nucleus. These data suggest that the nucleus stabilizes lead cell protrusions, helping to wedge open spaces between nurse cells.
Skip Nav Destination
Article navigation
6 November 2023
Article|
September 11 2023
Nuclear lamin facilitates collective border cell invasion into confined spaces in vivo
Lauren Penfield
,
(Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Software, Validation, Visualization, Writing - original draft, Writing - review & editing)
1Department of Molecular, Cellular, and Developmental Biology,
University of California Santa Barbara
, Santa Barbara, CA, USA
Lauren Penfield: laurenpenfield@ucsb.edu
Search for other works by this author on:
Denise J. Montell
(Conceptualization, Data curation, Funding acquisition, Methodology, Project administration, Resources, Supervision, Visualization, Writing - original draft, Writing - review & editing)
1Department of Molecular, Cellular, and Developmental Biology,
University of California Santa Barbara
, Santa Barbara, CA, USA
Correspondence to Denise J. Montell: dmontell@ucsb.edu
Search for other works by this author on:
Lauren Penfield
Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Software, Validation, Visualization, Writing - original draft, Writing - review & editing
1Department of Molecular, Cellular, and Developmental Biology,
University of California Santa Barbara
, Santa Barbara, CA, USA
Denise J. Montell
Conceptualization, Data curation, Funding acquisition, Methodology, Project administration, Resources, Supervision, Visualization, Writing - original draft, Writing - review & editing
1Department of Molecular, Cellular, and Developmental Biology,
University of California Santa Barbara
, Santa Barbara, CA, USA
Correspondence to Denise J. Montell: dmontell@ucsb.edu
Lauren Penfield: laurenpenfield@ucsb.edu
Disclosures: The authors declare no competing interests exist.
Received:
December 20 2022
Revision Received:
June 05 2023
Accepted:
August 11 2023
Online ISSN: 1540-8140
Print ISSN: 0021-9525
Funding
Funder(s):
National Institutes of Health
- Award Id(s): R01GM073164
Funder(s):
American Cancer Society
- Award Id(s): PF-22-091-01-MM
© 2023 Penfield and Montell
2023
Penfield and Montell
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
J Cell Biol (2023) 222 (11): e202212101.
Article history
Received:
December 20 2022
Revision Received:
June 05 2023
Accepted:
August 11 2023
Citation
Lauren Penfield, Denise J. Montell; Nuclear lamin facilitates collective border cell invasion into confined spaces in vivo. J Cell Biol 6 November 2023; 222 (11): e202212101. doi: https://doi.org/10.1083/jcb.202212101
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
Advertisement