The target of rapamycin complex 1 (TORC1) is mainly localized to the vacuolar membrane and regulates eukaryotic cell growth in response to nutrient availability. To obtain deeper insights into the functional roles of TORC1, we performed a genome-wide analysis of the TORC1 interactome in yeast using the bimolecular fluorescence complementation (BiFC) assay. We found that while most of the BiFC signals are observed at the vacuolar membrane, a fraction of them are detected at cytoplasmic messenger ribonucleoprotein (mRNP) granules. Moreover, mRNA-binding proteins are enriched in the TORC1 interactome, suggesting a functional relationship between TORC1 and mRNA metabolism. We show that a portion of TORC1 is consistently associated with mRNP complexes and interacts with a specific subset of mRNAs. We also demonstrate that TORC1 directly targets a translational repressor Scd6 and that the activity of Scd6 is inhibited by TORC1-dependent phosphorylation. Collectively, our data suggest that TORC1 plays a novel role in posttranscriptional regulation by controlling the activity of Scd6.
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February 10 2021
Analysis of the TORC1 interactome reveals a spatially distinct function of TORC1 in mRNP complexes
Yeonji Chang
,
Yeonji Chang
1
School of Biological Sciences, Seoul National University, Seoul, Republic of Korea
2
Institute of Microbiology, Seoul National University, Seoul, Republic of Korea
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Gyubum Lim
,
Gyubum Lim
1
School of Biological Sciences, Seoul National University, Seoul, Republic of Korea
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Won-Ki Huh
1
School of Biological Sciences, Seoul National University, Seoul, Republic of Korea
2
Institute of Microbiology, Seoul National University, Seoul, Republic of Korea
Correspondence to Won-Ki Huh: wkh@snu.ac.kr
Search for other works by this author on:
Yeonji Chang
1
School of Biological Sciences, Seoul National University, Seoul, Republic of Korea
2
Institute of Microbiology, Seoul National University, Seoul, Republic of Korea
Gyubum Lim
1
School of Biological Sciences, Seoul National University, Seoul, Republic of Korea
Won-Ki Huh
1
School of Biological Sciences, Seoul National University, Seoul, Republic of Korea
2
Institute of Microbiology, Seoul National University, Seoul, Republic of Korea
Correspondence to Won-Ki Huh: wkh@snu.ac.kr
Received:
December 11 2019
Revision Received:
November 15 2020
Accepted:
January 06 2021
Online Issn: 1540-8140
Print Issn: 0021-9525
Funding:
Ministry of Education, Science and Technology
(NO AWARD)
National Research Foundation of Korea
(2015R1A2A1A01007871)
© 2021 Chang et al.
2021
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
J Cell Biol (2021) 220 (4): e201912060.
Article history
Received:
December 11 2019
Revision Received:
November 15 2020
Accepted:
January 06 2021
Citation
Yeonji Chang, Gyubum Lim, Won-Ki Huh; Analysis of the TORC1 interactome reveals a spatially distinct function of TORC1 in mRNP complexes. J Cell Biol 5 April 2021; 220 (4): e201912060. doi: https://doi.org/10.1083/jcb.201912060
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