The spindle pole body (SPB) provides microtubule-organizing functions in yeast and duplicates exactly once per cell cycle. The first step in SPB duplication is the half-bridge to bridge conversion via the antiparallel dimerization of the centrin (Cdc31)-binding protein Sfi1 in anaphase. The bridge, which is anchored to the old SPB on the proximal end, exposes free Sfi1 N-termini (N-Sfi1) at its distal end. These free N-Sfi1 promote in G1 the assembly of the daughter SPB (dSPB) in a yet unclear manner. This study shows that N-Sfi1 including the first three Cdc31 binding sites interacts with the SPB components Spc29 and Spc42, triggering the assembly of the dSPB. Cdc31 binding to N-Sfi1 promotes Spc29 recruitment and is essential for satellite formation. Furthermore, phosphorylation of N-Sfi1 has an inhibitory effect and delays dSPB biogenesis until G1. Taking these data together, we provide an understanding of the initial steps in SPB assembly and describe a new function of Cdc31 in the recruitment of dSPB components.
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1 March 2021
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February 01 2021
The N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body
Diana Rüthnick
,
Diana Rüthnick
*
Center for Molecular Biology, University of Heidelberg, German Cancer Research Center–Center for Molecular Biology Alliance, Heidelberg, Germany
Correspondence to Diana Rüthnick: d.ruethnick@zmbh.uni-heidelberg.de
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Jlenia Vitale
,
Jlenia Vitale
*
Center for Molecular Biology, University of Heidelberg, German Cancer Research Center–Center for Molecular Biology Alliance, Heidelberg, Germany
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Annett Neuner
,
Annett Neuner
Center for Molecular Biology, University of Heidelberg, German Cancer Research Center–Center for Molecular Biology Alliance, Heidelberg, Germany
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Elmar Schiebel
Center for Molecular Biology, University of Heidelberg, German Cancer Research Center–Center for Molecular Biology Alliance, Heidelberg, Germany
Elmar Schiebel: e.schiebel@zmbh.uni-heidelbeg.de
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Diana Rüthnick
Center for Molecular Biology, University of Heidelberg, German Cancer Research Center–Center for Molecular Biology Alliance, Heidelberg, Germany
Jlenia Vitale
Center for Molecular Biology, University of Heidelberg, German Cancer Research Center–Center for Molecular Biology Alliance, Heidelberg, Germany
Annett Neuner
Center for Molecular Biology, University of Heidelberg, German Cancer Research Center–Center for Molecular Biology Alliance, Heidelberg, Germany
Elmar Schiebel
Center for Molecular Biology, University of Heidelberg, German Cancer Research Center–Center for Molecular Biology Alliance, Heidelberg, Germany
*
D. Rüthnick and J. Vitale contributed equally to this paper.
Elmar Schiebel: e.schiebel@zmbh.uni-heidelbeg.de
Correspondence to Diana Rüthnick: d.ruethnick@zmbh.uni-heidelberg.de
Received:
April 27 2020
Revision Received:
November 20 2020
Accepted:
December 18 2020
Online Issn: 1540-8140
Print Issn: 0021-9525
Funding:
Deutsche Forschungsgemeinschaft
(Schi 295/5-3)
© 2021 Rüthnick et al.
2021
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
J Cell Biol (2021) 220 (3): e202004196.
Article history
Received:
April 27 2020
Revision Received:
November 20 2020
Accepted:
December 18 2020
Citation
Diana Rüthnick, Jlenia Vitale, Annett Neuner, Elmar Schiebel; The N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body. J Cell Biol 1 March 2021; 220 (3): e202004196. doi: https://doi.org/10.1083/jcb.202004196
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