During centriole duplication, a preprocentriole forms at a single site on the mother centriole through a process that includes the hierarchical recruitment of a conserved set of proteins, including the Polo-like kinase 4 (Plk4), Ana2/STIL, and the cartwheel protein Sas6. Ana2/STIL is critical for procentriole assembly, and its recruitment is controlled by the kinase activity of Plk4, but how this works remains poorly understood. A structural motif called the G-box in the centriole outer wall protein Sas4 interacts with a short region in the N terminus of Ana2/STIL. Here, we show that binding of Ana2 to the Sas4 G-box enables hyperphosphorylation of the Ana2 N terminus by Plk4. Hyperphosphorylation increases the affinity of the Ana2–G-box interaction, and, consequently, promotes the accumulation of Ana2 at the procentriole to induce daughter centriole formation.
A molecular mechanism for the procentriole recruitment of Ana2
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Tiffany A. McLamarrah, Sarah K. Speed, John M. Ryniawec, Daniel W. Buster, Carey J. Fagerstrom, Brian J. Galletta, Nasser M. Rusan, Gregory C. Rogers; A molecular mechanism for the procentriole recruitment of Ana2. J Cell Biol 3 February 2020; 219 (2): e201905172. doi: https://doi.org/10.1083/jcb.201905172
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