Many tissues are produced by specialized progenitor cells emanating from epithelia via epithelial-to-mesenchymal transition (EMT). Most studies have so far focused on EMT involving single or isolated groups of cells. Here we describe an EMT-like process that requires tissue-level coordination. This EMT-like process occurs along a continuous front in the Drosophila optic lobe neuroepithelium to produce neural stem cells (NSCs). We find that emerging NSCs remain epithelial and apically constrict before dividing asymmetrically to produce neurons. Apical constriction is associated with contractile myosin pulses and involves RhoGEF3 and down-regulation of the Crumbs complex by the E3 ubiquitin ligase Neuralized. Anisotropy in Crumbs complex levels also results in accumulation of junctional myosin. Disrupting the regulation of Crumbs by Neuralized lowered junctional myosin and led to imprecision in the integration of emerging NSCs into the front. Thus, Neuralized promotes smooth progression of the differentiation front by coupling epithelium remodeling at the tissue level with NSC fate acquisition.
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2 November 2020
Article|
September 18 2020
Tissue-wide coordination of epithelium-to-neural stem cell transition in the Drosophila optic lobe requires Neuralized
In Special Collection:
Cellular Neurobiology 2020
Chloé Shard,
Chloé Shard
1
Institut Pasteur, Paris, France
2
UMR3738, Centre National de la Recherche Scientifique, Paris, France
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Juan Luna-Escalante,
Juan Luna-Escalante
1
Institut Pasteur, Paris, France
2
UMR3738, Centre National de la Recherche Scientifique, Paris, France
3
Laboratoire de Physique, Ecole Normale Supérieure, Centre National de la Recherche Scientifique, Sorbonne Université, Université Paris Diderot, Paris, France
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François Schweisguth
1
Institut Pasteur, Paris, France
2
UMR3738, Centre National de la Recherche Scientifique, Paris, France
Correspondence to François Schweisguth: fschweis@pasteur.fr
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Chloé Shard
1
Institut Pasteur, Paris, France
2
UMR3738, Centre National de la Recherche Scientifique, Paris, France
Juan Luna-Escalante
1
Institut Pasteur, Paris, France
2
UMR3738, Centre National de la Recherche Scientifique, Paris, France
3
Laboratoire de Physique, Ecole Normale Supérieure, Centre National de la Recherche Scientifique, Sorbonne Université, Université Paris Diderot, Paris, France
François Schweisguth
1
Institut Pasteur, Paris, France
2
UMR3738, Centre National de la Recherche Scientifique, Paris, France
Correspondence to François Schweisguth: fschweis@pasteur.fr
Received:
May 06 2020
Revision Received:
August 06 2020
Accepted:
August 17 2020
Online Issn: 1540-8140
Print Issn: 0021-9525
© 2020 Shard et al.
2020
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
J Cell Biol (2020) 219 (11): e202005035.
Article history
Received:
May 06 2020
Revision Received:
August 06 2020
Accepted:
August 17 2020
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Citation
Chloé Shard, Juan Luna-Escalante, François Schweisguth; Tissue-wide coordination of epithelium-to-neural stem cell transition in the Drosophila optic lobe requires Neuralized. J Cell Biol 2 November 2020; 219 (11): e202005035. doi: https://doi.org/10.1083/jcb.202005035
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