We examined post-eclosion elimination of the Drosophila wing epithelium in vivo where collective “suicide waves” promote sudden, coordinated death of epithelial sheets without a final engulfment step. Like apoptosis in earlier developmental stages, this unique communal form of cell death is controlled through the apoptosome proteins, Dronc and Dark, together with the IAP antagonists, Reaper, Grim, and Hid. Genetic lesions in these pathways caused intervein epithelial cells to persist, prompting a characteristic late-onset blemishing phenotype throughout the wing blade. We leveraged this phenotype in mosaic animals to discover relevant genes and establish here that homeodomain interacting protein kinase (HIPK) is required for collective death of the wing epithelium. Extra cells also persisted in other tissues, establishing a more generalized requirement for HIPK in the regulation of cell death and cell numbers.
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13 August 2007
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August 06 2007
A collective form of cell death requires homeodomain interacting protein kinase
Nichole Link,
Nichole Link
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
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Po Chen,
Po Chen
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
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Wan-Jin Lu,
Wan-Jin Lu
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
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Kristi Pogue,
Kristi Pogue
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
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Amy Chuong,
Amy Chuong
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
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Miguel Mata,
Miguel Mata
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
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Joshua Checketts,
Joshua Checketts
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
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John M. Abrams
John M. Abrams
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
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Nichole Link
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
Po Chen
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
Wan-Jin Lu
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
Kristi Pogue
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
Amy Chuong
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
Miguel Mata
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
Joshua Checketts
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
John M. Abrams
Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390
Correspondence to John Abrams: [email protected]
N. Link and P. Chen contributed equally to this paper.
Abbreviations used in this paper: FLP, flippase; FRT, FLP recombinase target; HIPK, homeodomain interacting protein kinase; PCD, programmed cell death WT, wild type.
Received:
February 20 2007
Accepted:
July 11 2007
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2007
J Cell Biol (2007) 178 (4): 567–574.
Article history
Received:
February 20 2007
Accepted:
July 11 2007
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Citation
Nichole Link, Po Chen, Wan-Jin Lu, Kristi Pogue, Amy Chuong, Miguel Mata, Joshua Checketts, John M. Abrams; A collective form of cell death requires homeodomain interacting protein kinase . J Cell Biol 13 August 2007; 178 (4): 567–574. doi: https://doi.org/10.1083/jcb.200702125
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