Ubiquitin flips the switch for spindle checkpoint inactivation, according to a pair of studies by Sashank Reddy, Marc Kirschner, and colleagues and by Frank Stegmeier, Stephen Elledge (Harvard Medical School, Boston, MA), and colleagues.
The checkpoint stands guard over mitosis to ensure accurate chromosome segregation. While the spindle takes time to assemble, two checkpoint proteins bind tightly to Cdc20 to keep it from activating the anaphase-promoting complex (APC). The checkpoint stays on as long as it senses that kinetochores are still unattached.
The researchers found that flooding the system with UbcH10, which ubiquitinates APC, or p31comet, a protein previously implicated in checkpoint inactivation, caused cells to crash through the checkpoint. These proteins promoted APC-dependent ubiquitination of Cdc20, which pried it from the checkpoint proteins' grip and released the brake on anaphase.
An RNAi screen in the Elledge lab uncovered a deubiquitinating enzyme, called...
