The epithelial cell–specific adaptor complex AP-1B is crucial for correct delivery of many transmembrane proteins from recycling endosomes to the basolateral plasma membrane. Subsequently, membrane fusion is dependent on the formation of complexes between SNARE proteins located at the target membrane and on transport vesicles. Although the t-SNARE syntaxin 4 has been localized to the basolateral membrane, the v-SNARE operative in the AP-1B pathway remained unknown. We show that the ubiquitously expressed v-SNARE cellubrevin localizes to the basolateral membrane and to recycling endosomes, where it colocalizes with AP-1B. Furthermore, we demonstrate that cellubrevin coimmunoprecipitates preferentially with syntaxin 4, implicating this v-SNARE in basolateral fusion events. Cleavage of cellubrevin with tetanus neurotoxin (TeNT) results in scattering of AP-1B localization and missorting of AP-1B–dependent cargos, such as transferrin receptor and a truncated low-density lipoprotein receptor, LDLR-CT27. These data suggest that cellubrevin and AP-1B cooperate in basolateral membrane trafficking.
Skip Nav Destination
Article navigation
7 May 2007
Article|
May 07 2007
v-SNARE cellubrevin is required for basolateral sorting of AP-1B–dependent cargo in polarized epithelial cells
Ian C. Fields,
Ian C. Fields
1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208
Search for other works by this author on:
Elina Shteyn,
Elina Shteyn
1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208
Search for other works by this author on:
Marc Pypaert,
Marc Pypaert
2Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
Search for other works by this author on:
Véronique Proux-Gillardeaux,
Véronique Proux-Gillardeaux
3Institut National de la Santé et de la Recherche Médicale Avenir Team, Institut Jacques Monod, Unité Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Universities Paris 6 and Paris 7, F-75005 Paris, Cedex 05, France
Search for other works by this author on:
Richard S. Kang,
Richard S. Kang
1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208
Search for other works by this author on:
Thierry Galli,
Thierry Galli
3Institut National de la Santé et de la Recherche Médicale Avenir Team, Institut Jacques Monod, Unité Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Universities Paris 6 and Paris 7, F-75005 Paris, Cedex 05, France
Search for other works by this author on:
Heike Fölsch
Heike Fölsch
1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208
Search for other works by this author on:
Ian C. Fields
1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208
Elina Shteyn
1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208
Marc Pypaert
2Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
Véronique Proux-Gillardeaux
3Institut National de la Santé et de la Recherche Médicale Avenir Team, Institut Jacques Monod, Unité Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Universities Paris 6 and Paris 7, F-75005 Paris, Cedex 05, France
Richard S. Kang
1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208
Thierry Galli
3Institut National de la Santé et de la Recherche Médicale Avenir Team, Institut Jacques Monod, Unité Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Universities Paris 6 and Paris 7, F-75005 Paris, Cedex 05, France
Heike Fölsch
1Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208
Correspondence to Heike Fölsch: [email protected]
Abbreviations used in this paper: AP, adaptor protein; FcR, FcII-B2 receptor; LDLR, low-density lipoprotein receptor; TeNT, tetanus neurotoxin; TfnR, transferrin receptor; VAMP, vesicle-associated membrane protein; VSVG, vesicular stomatitis virus glycoprotein.
Received:
October 10 2006
Accepted:
April 05 2007
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2007
J Cell Biol (2007) 177 (3): 477–488.
Article history
Received:
October 10 2006
Accepted:
April 05 2007
Citation
Ian C. Fields, Elina Shteyn, Marc Pypaert, Véronique Proux-Gillardeaux, Richard S. Kang, Thierry Galli, Heike Fölsch; v-SNARE cellubrevin is required for basolateral sorting of AP-1B–dependent cargo in polarized epithelial cells . J Cell Biol 7 May 2007; 177 (3): 477–488. doi: https://doi.org/10.1083/jcb.200610047
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionSuggested Content
Email alerts
Advertisement
Advertisement