Autophagy is a conserved degradative pathway that is induced in response to various stress and developmental conditions in eukaryotic cells. It allows the elimination of cytosolic proteins and organelles in the lysosome/vacuole. In the yeast Saccharomyces cerevisiae, the integral membrane protein Atg9 (autophagy-related protein 9) cycles between mitochondria and the preautophagosomal structure (PAS), the nucleating site for formation of the sequestering vesicle, suggesting a role in supplying membrane for vesicle formation and/or expansion during autophagy. To better understand the mechanisms involved in Atg9 cycling, we performed a yeast two-hybrid–based screen and identified a peripheral membrane protein, Atg11, that interacts with Atg9. We show that Atg11 governs Atg9 cycling through the PAS during specific autophagy. We also demonstrate that the integrity of the actin cytoskeleton is essential for correct targeting of Atg11 to the PAS. We propose that a pool of Atg11 mediates the anterograde transport of Atg9 to the PAS that is dependent on the actin cytoskeleton during yeast vegetative growth.
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18 December 2006
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December 18 2006
Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast
Congcong He,
Congcong He
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
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Hui Song,
Hui Song
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
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Tomohiro Yorimitsu,
Tomohiro Yorimitsu
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
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Iryna Monastyrska,
Iryna Monastyrska
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
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Wei-Lien Yen,
Wei-Lien Yen
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
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Julie E. Legakis,
Julie E. Legakis
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
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Daniel J. Klionsky
Daniel J. Klionsky
1Department of Molecular, Cellular, and Developmental Biology
2Department of Biological Chemistry,
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
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Congcong He
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
Hui Song
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
Tomohiro Yorimitsu
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
Iryna Monastyrska
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
Wei-Lien Yen
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
Julie E. Legakis
1Department of Molecular, Cellular, and Developmental Biology
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
Daniel J. Klionsky
1Department of Molecular, Cellular, and Developmental Biology
2Department of Biological Chemistry,
3Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109
Correspondence to Daniel J. Klionsky: [email protected]
Abbreviations used in this paper: AD, activation domain; Ape1, aminopeptidase I; Atg, autophagy related; BD, binding domain; BFP, blue fluorescent protein; CC, coiled coil; Cvt, cytoplasm to vacuole targeting; PA, protein A; PAS, preautophagosomal structure; prApe1, precursor form of Ape1; SMD, synthetic minimal medium.
Received:
June 16 2006
Accepted:
November 16 2006
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2006
J Cell Biol (2006) 175 (6): 925–935.
Article history
Received:
June 16 2006
Accepted:
November 16 2006
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Citation
Congcong He, Hui Song, Tomohiro Yorimitsu, Iryna Monastyrska, Wei-Lien Yen, Julie E. Legakis, Daniel J. Klionsky; Recruitment of Atg9 to the preautophagosomal structure by Atg11 is essential for selective autophagy in budding yeast . J Cell Biol 18 December 2006; 175 (6): 925–935. doi: https://doi.org/10.1083/jcb.200606084
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