Accurate chromosome segregation during mitosis requires biorientation of sister chromatids on the microtubules (MT) of the mitotic spindle. Chromosome–MT binding is mediated by kinetochores, which are multiprotein structures that assemble on centromeric (CEN) DNA. The simple CENs of budding yeast are among the best understood, but the roles of kinesin motor proteins at yeast kinetochores have yet to be determined, despite evidence of their importance in higher eukaryotes. We show that all four nuclear kinesins in Saccharomyces cerevisiae localize to kinetochores and function in three distinct processes. Kip1p and Cin8p, which are kinesin-5/BimC family members, cluster kinetochores into their characteristic bilobed metaphase configuration. Kip3p, a kinesin-8,-13/KinI kinesin, synchronizes poleward kinetochore movement during anaphase A. The kinesin-14 motor Kar3p appears to function at the subset of kinetochores that become detached from spindle MTs. These data demonstrate roles for structurally diverse motors in the complex processes of chromosome segregation and reveal important similarities and intriguing differences between higher and lower eukaryotes.
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13 March 2006
Article|
March 13 2006
Analysis of kinesin motor function at budding yeast kinetochores
Jessica D. Tytell,
Jessica D. Tytell
1Department of Biology
Search for other works by this author on:
Peter K. Sorger
Peter K. Sorger
1Department of Biology
2Biological Engineering Division Massachusetts Institute of Technology Cambridge MA 02139
Search for other works by this author on:
Jessica D. Tytell
1Department of Biology
Peter K. Sorger
1Department of Biology
2Biological Engineering Division Massachusetts Institute of Technology Cambridge MA 02139
Correspondence to Peter K. Sorger: [email protected]
Abbreviations used in this paper: 2D, two-dimensional; 3D, three-dimensional; CEN, centromeric; ChIP, chromatin immunoprecipitation; kMT, kinetochore microtubule; MAP, MT-associated protein; MT, microtubules; SPB, spindle pole body; pMT, pole-to-pole MTs.
Received:
September 16 2005
Accepted:
February 09 2006
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2006
J Cell Biol (2006) 172 (6): 861–874.
Article history
Received:
September 16 2005
Accepted:
February 09 2006
Citation
Jessica D. Tytell, Peter K. Sorger; Analysis of kinesin motor function at budding yeast kinetochores . J Cell Biol 13 March 2006; 172 (6): 861–874. doi: https://doi.org/10.1083/jcb.200509101
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