Multiple mitotic kinesins and microtubule-associated proteins (MAPs) act in concert to direct cytokinesis (Glotzer, M. 2005. Science. 307:1735–1739). In anaphase cells, many of these proteins associate with an antiparallel array of microtubules termed the central spindle. The MAP and microtubule-bundling protein PRC1 (protein-regulating cytokinesis 1) is one of the key molecules required for the integrity of this structure (Jiang, W., G. Jimenez, N.J. Wells, T.J. Hope, G.M. Wahl, T. Hunter, and R. Fukunaga. 1998. Mol. Cell. 2:877–885; Mollinari, C., J.P. Kleman, W. Jiang, G. Schoehn, T. Hunter, and R.L. Margolis. 2002. J. Cell Biol. 157:1175–1186). In this study, we identify an interaction between endogenous PRC1 and the previously uncharacterized kinesin KIF14 as well as other mitotic kinesins (MKlp1/CHO1, MKlp2, and KIF4) with known functions in cytokinesis (Hill, E., M. Clarke, and F.A. Barr. 2000. EMBO J. 19:5711–5719; Matuliene, J., and R. Kuriyama. 2002. Mol. Biol. Cell. 13:1832–1845; Kurasawa, Y., W.C. Earnshaw, Y. Mochizuki, N. Dohmae, and K. Todokoro. 2004. EMBO J. 23:3237–3248). We find that KIF14 targets to the central spindle via its interaction with PRC1 and has an essential function in cytokinesis. In KIF14-depleted cells, citron kinase but not other components of the central spindle and cleavage furrow fail to localize. Furthermore, the localization of KIF14 and citron kinase to the central spindle and midbody is codependent, and they form a complex depending on the activation state of citron kinase. Contrary to a previous study (Di Cunto, F., S. Imarisio, E. Hirsch, V. Broccoli, A. Bulfone, A. Migheli, C. Atzori, E. Turco, R. Triolo, G.P. Dotto, et al. 2000. Neuron. 28:115–127), we find a general requirement for citron kinase in human cell division. Together, these findings identify a novel pathway required for efficient cytokinesis.
Skip Nav Destination
Article navigation
30 January 2006
Article|
January 23 2006
KIF14 and citron kinase act together to promote efficient cytokinesis
Ulrike Gruneberg,
Ulrike Gruneberg
1Department of Cell Biology
Search for other works by this author on:
Rüdiger Neef,
Rüdiger Neef
2Intracellular Protein Transport Group, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
Search for other works by this author on:
Eunice H.Y. Chan,
Eunice H.Y. Chan
1Department of Cell Biology
Search for other works by this author on:
Ravindra B. Chalamalasetty,
Ravindra B. Chalamalasetty
1Department of Cell Biology
Search for other works by this author on:
Francis A. Barr
Francis A. Barr
2Intracellular Protein Transport Group, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
Search for other works by this author on:
Ulrike Gruneberg
1Department of Cell Biology
Rüdiger Neef
2Intracellular Protein Transport Group, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
Xiuling Li
1Department of Cell Biology
Eunice H.Y. Chan
1Department of Cell Biology
Ravindra B. Chalamalasetty
1Department of Cell Biology
Erich A. Nigg
1Department of Cell Biology
Francis A. Barr
2Intracellular Protein Transport Group, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
Correspondence to Francis A. Barr: [email protected]
Abbreviations used in this paper: FHA, forkhead associated; INCENP, inner centromere protein; MAP, microtubule-associated protein; PRC1, protein-regulating cytokinesis 1; siRNA, small interfering RNA.
Received:
November 15 2005
Accepted:
December 27 2005
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2006
J Cell Biol (2006) 172 (3): 363–372.
Article history
Received:
November 15 2005
Accepted:
December 27 2005
Citation
Ulrike Gruneberg, Rüdiger Neef, Xiuling Li, Eunice H.Y. Chan, Ravindra B. Chalamalasetty, Erich A. Nigg, Francis A. Barr; KIF14 and citron kinase act together to promote efficient cytokinesis . J Cell Biol 30 January 2006; 172 (3): 363–372. doi: https://doi.org/10.1083/jcb.200511061
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement