Rab guanosine triphosphatases regulate intracellular membrane traffic by binding specific effector proteins. The yeast Rab Sec4p plays multiple roles in the polarized transport of post-Golgi vesicles to, and their subsequent fusion with, the plasma membrane, suggesting the involvement of several effectors. Yet, only one Sec4p effector has been documented to date: the exocyst protein Sec15p. The exocyst is an octameric protein complex required for tethering secretory vesicles, which is a prerequisite for membrane fusion. In this study, we describe the identification of a second Sec4p effector, Sro7p, which is a member of the lethal giant larvae tumor suppressor family. Sec4-GTP binds to Sro7p in cell extracts as well as to purified Sro7p, and the two proteins can be coimmunoprecipitated. Furthermore, we demonstrate the formation of a ternary complex of Sec4-GTP, Sro7p, and the t-SNARE Sec9p. Genetic data support our conclusion that Sro7p functions downstream of Sec4p and further imply that Sro7p and the exocyst share partially overlapping functions, possibly in SNARE regulation.
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2 January 2006
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January 03 2006
The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p
Bianka L. Grosshans,
Bianka L. Grosshans
1Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
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Anna Andreeva,
Anna Andreeva
2Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
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Akanksha Gangar,
Akanksha Gangar
2Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
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Sherry Niessen,
Sherry Niessen
3Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037
4Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037
5The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037
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John R. Yates, III,
John R. Yates, III
3Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037
4Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037
5The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037
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Patrick Brennwald,
Patrick Brennwald
2Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
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Peter Novick
Peter Novick
1Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
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Bianka L. Grosshans
1Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
Anna Andreeva
2Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
Akanksha Gangar
2Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
Sherry Niessen
3Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037
4Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037
5The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037
John R. Yates, III
3Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037
4Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037
5The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037
Patrick Brennwald
2Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
Peter Novick
1Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
Correspondence to Peter Novick: [email protected]
Abbreviations used in this paper: GEF, guanine nucleotide exchange factor; GDP, guanosine 5′-diphosphate; lgl, lethal giant larvae; SC, synthetic complete; YPD, yeast peptone dextrose.
Received:
October 04 2005
Accepted:
November 22 2005
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2006
J Cell Biol (2006) 172 (1): 55–66.
Article history
Received:
October 04 2005
Accepted:
November 22 2005
Citation
Bianka L. Grosshans, Anna Andreeva, Akanksha Gangar, Sherry Niessen, John R. Yates, Patrick Brennwald, Peter Novick; The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p . J Cell Biol 2 January 2006; 172 (1): 55–66. doi: https://doi.org/10.1083/jcb.200510016
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