The origin recognition complex (ORC) ensures exactly one round of genome replication per cell cycle through acting as a molecular switch that precisely controls the assembly, firing, and inactivation of the replication initiation machinery. Recent data indicate that it may also coordinate the processes of mitosis and cytokinesis and ensure the proper distribution of replicated genome to daughter cells. We have found that the ORC core subunits are highly expressed in the nervous system. They are selectively localized to the neuronal somatodendritic compartment and enriched in the membrane fraction. siRNA knockdown of ORC subunits dramatically reduced dendritic branch formation and severely impeded dendritic spine emergence. Expression of ORC ATPase motif mutants enhanced the branching of dendritic arbors. The ORC core complex thus appears to have a novel role in regulating dendrite and dendritic spine development in postmitotic neurons.
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15 August 2005
Article|
August 08 2005
The origin recognition core complex regulates dendrite and spine development in postmitotic neurons
Zhen Huang,
Zhen Huang
1Department of Physiology, University of California San Francisco, San Francisco, CA 94143
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Keling Zang,
Keling Zang
2Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA 94143
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Louis F. Reichardt
Louis F. Reichardt
1Department of Physiology, University of California San Francisco, San Francisco, CA 94143
2Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA 94143
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Zhen Huang
1Department of Physiology, University of California San Francisco, San Francisco, CA 94143
Keling Zang
2Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA 94143
Louis F. Reichardt
1Department of Physiology, University of California San Francisco, San Francisco, CA 94143
2Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA 94143
Correspondence to Louis F. Reichardt: [email protected]; or Zhen Huang: [email protected]
Abbreviations used in this paper: DIV, days in vitro; NMJ, neuromuscular junction; ORC, origin recognition complex.
This article contains online supplemental material.
Received:
May 12 2005
Accepted:
June 29 2005
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2005
J Cell Biol (2005) 170 (4): 527–535.
Article history
Received:
May 12 2005
Accepted:
June 29 2005
Citation
Zhen Huang, Keling Zang, Louis F. Reichardt; The origin recognition core complex regulates dendrite and spine development in postmitotic neurons . J Cell Biol 15 August 2005; 170 (4): 527–535. doi: https://doi.org/10.1083/jcb.200505075
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