Runt RNA (red) disperses (left to right) after injection of anti-dynein antibody.

DAVIS/ELSEVIER

Dynein, well-known for moving cargo down microtubules, is now also shown to anchor its cargo at the target site, according to results from Renald Delanoue and Ilan Davis (University of Edinburgh, UK).

Dynein's cargoes include the wingless and runt mRNAs, which concentrate at the apical tip of fly embryos. Delanoue and Davis found that agents that disrupt microtubule function caused the mRNAs to disperse. Injection of antibodies against dynein subunits disrupted localization of both injected exogenous RNAs and endogenous transcripts, indicating that dynein itself was doing the anchoring.To distinguish between static anchoring and continuous localization, the team used a two-step injection design. They injected one batch of labeled RNA, allowed it to localize, and then injected a second batch along with an ATPase inhibitor to block dynein motility. Under these conditions, the...

The Rockefeller University Press
2005
The Rockefeller University Press
2005
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