Choose the right experimental system to test a hypothesis: that has and always will be one of the ten commandments of cell biology. By the mid-1960s, several secretory cell types—liver, pancreatic, pituitary—had been exploited for tracking the path and fate of their abundant loads of secreted proteins. And by 1964, these cells had revealed a sketch of the secretory pathway from the ER to the Golgi, to vesicles, and out to the cellular membrane. This was most clearly seen when Caro and Palade (1964) combined autoradiography and electron microscopy to trace radioactive leucine in pancreatic cells.

Labeling of the distinctive Golgi in goblet cells identifies it as the site of glycosylation.

NEUTRA

But carbohydrates were not part of the picture, at least partly because the secretion systems studied up to that point pumped out poorly glycosylated proteins. So Marian Neutra (now at Harvard Medical School,...

The Rockefeller University Press
2005
The Rockefeller University Press
2005
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