Recent studies have revealed an important role for tight junction protein complexes in epithelial cell polarity. One of these complexes contains the apical transmembrane protein, Crumbs, and two PSD95/discs large/zonula occludens domain proteins, protein associated with Lin seven 1 (PALS1)/Stardust and PALS1-associated tight junction protein (PATJ). Although Crumbs and PALS1/Stardust are known to be important for cell polarization, recent studies have suggested that Drosophila PATJ is not essential and its function is unclear. Here, we find that PATJ is targeted to the apical region and tight junctions once cell polarization is initiated. We show using RNAi techniques that reduction in PATJ expression leads to delayed tight junction formation as well as defects in cell polarization. These effects are reversed by reintroduction of PATJ into these RNAi cells. This study provides new functional information on PATJ as a polarity protein and increases our understanding of the Crumbs–PALS1–PATJ complex function in epithelial polarity.
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28 February 2005
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February 28 2005
PATJ regulates tight junction formation and polarity in mammalian epithelial cells
Kunyoo Shin,
Kunyoo Shin
1Department of Biological Chemistry, University of Michigan Medical School Ann Arbor, MI 48109
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Sam Straight,
Sam Straight
3Howard Hughes Medical Institute, University of Michigan Medical School Ann Arbor, MI 48109
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Ben Margolis
Ben Margolis
1Department of Biological Chemistry, University of Michigan Medical School Ann Arbor, MI 48109
2Department of Internal Medicine, University of Michigan Medical School Ann Arbor, MI 48109
3Howard Hughes Medical Institute, University of Michigan Medical School Ann Arbor, MI 48109
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Kunyoo Shin
1Department of Biological Chemistry, University of Michigan Medical School Ann Arbor, MI 48109
Sam Straight
3Howard Hughes Medical Institute, University of Michigan Medical School Ann Arbor, MI 48109
Ben Margolis
1Department of Biological Chemistry, University of Michigan Medical School Ann Arbor, MI 48109
2Department of Internal Medicine, University of Michigan Medical School Ann Arbor, MI 48109
3Howard Hughes Medical Institute, University of Michigan Medical School Ann Arbor, MI 48109
Correspondence to Ben Margolis: [email protected]
Abbreviations used in this paper: CRB3, Crumbs3; DM-PATJ, Drosophila PATJ; L27, Lin-2 and Lin-7; PALS1, protein associated with Lin seven 1; PATJ, PALS1-associated tight junction protein; PDZ, PSD95/discs large/zonula occludens; TER, transepithelial electrical resistance; VAC, vacuolar apical compartment; ZO, zonula occludens.
Received:
August 10 2004
Accepted:
December 28 2004
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2005
J Cell Biol (2005) 168 (5): 705–711.
Article history
Received:
August 10 2004
Accepted:
December 28 2004
Citation
Kunyoo Shin, Sam Straight, Ben Margolis; PATJ regulates tight junction formation and polarity in mammalian epithelial cells . J Cell Biol 28 February 2005; 168 (5): 705–711. doi: https://doi.org/10.1083/jcb.200408064
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