Neural activity induces the remodeling of pre- and postsynaptic membranes, which maintain their apposition through cell adhesion molecules. Among them, N-cadherin is redistributed, undergoes activity-dependent conformational changes, and is required for synaptic plasticity. Here, we show that depolarization induces the enlargement of the width of spine head, and that cadherin activity is essential for this synaptic rearrangement. Dendritic spines visualized with green fluorescent protein in hippocampal neurons showed an expansion by the activation of AMPA receptor, so that the synaptic apposition zone may be expanded. N-cadherin-venus fusion protein laterally dispersed along the expanding spine head. Overexpression of dominant-negative forms of N-cadherin resulted in the abrogation of the spine expansion. Inhibition of actin polymerization with cytochalasin D abolished the spine expansion. Together, our data suggest that cadherin-based adhesion machinery coupled with the actin-cytoskeleton is critical for the remodeling of synaptic apposition zone.
Skip Nav Destination
Article navigation
6 December 2004
Article|
November 29 2004
Cadherin activity is required for activity-induced spine remodeling
Ko Okamura,
Ko Okamura
1Department of Pharmacology, Osaka University Medical School, Suita, Osaka 565-0871 Japan
Search for other works by this author on:
Hidekazu Tanaka,
Hidekazu Tanaka
1Department of Pharmacology, Osaka University Medical School, Suita, Osaka 565-0871 Japan
Search for other works by this author on:
Yoshiki Yagita,
Yoshiki Yagita
2Department of Internal Medicine, Osaka University Medical School, Suita, Osaka 565-0871 Japan
3The Montreal Neurological Institute, Montreal, Quebec H3A 2B4
Search for other works by this author on:
Yoshinaga Saeki,
Yoshinaga Saeki
4Neuro-Oncology Laboratory, Massachusetts General Hospital, Charlestown, MA 02114
Search for other works by this author on:
Akihiko Taguchi,
Akihiko Taguchi
5National Cardiovascular Center, Suita, Osaka 565-8565 Japan
Search for other works by this author on:
Yasushi Hiraoka,
Yasushi Hiraoka
6Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492 Japan
Search for other works by this author on:
Ling-Hui Zeng,
Ling-Hui Zeng
1Department of Pharmacology, Osaka University Medical School, Suita, Osaka 565-0871 Japan
Search for other works by this author on:
David R Colman,
David R Colman
3The Montreal Neurological Institute, Montreal, Quebec H3A 2B4
Search for other works by this author on:
Naomasa Miki
Naomasa Miki
1Department of Pharmacology, Osaka University Medical School, Suita, Osaka 565-0871 Japan
Search for other works by this author on:
Ko Okamura
1Department of Pharmacology, Osaka University Medical School, Suita, Osaka 565-0871 Japan
Hidekazu Tanaka
1Department of Pharmacology, Osaka University Medical School, Suita, Osaka 565-0871 Japan
Yoshiki Yagita
2Department of Internal Medicine, Osaka University Medical School, Suita, Osaka 565-0871 Japan
3The Montreal Neurological Institute, Montreal, Quebec H3A 2B4
Yoshinaga Saeki
4Neuro-Oncology Laboratory, Massachusetts General Hospital, Charlestown, MA 02114
Akihiko Taguchi
5National Cardiovascular Center, Suita, Osaka 565-8565 Japan
Yasushi Hiraoka
6Kansai Advanced Research Center, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492 Japan
Ling-Hui Zeng
1Department of Pharmacology, Osaka University Medical School, Suita, Osaka 565-0871 Japan
David R Colman
3The Montreal Neurological Institute, Montreal, Quebec H3A 2B4
Naomasa Miki
1Department of Pharmacology, Osaka University Medical School, Suita, Osaka 565-0871 Japan
Correspondence to Hidekazu Tanaka: [email protected]
K. Okamura and H. Tanaka contributed equally to this work.
Abbreviations used in this paper: CCD, charge-coupled imaging device; DIV, day in vitro; SCCL, spine cotyloid curve length; W2A-cadherin, W2A mutant of N-cadherin.
Received:
June 04 2004
Accepted:
October 28 2004
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2004
J Cell Biol (2004) 167 (5): 961–972.
Article history
Received:
June 04 2004
Accepted:
October 28 2004
Citation
Ko Okamura, Hidekazu Tanaka, Yoshiki Yagita, Yoshinaga Saeki, Akihiko Taguchi, Yasushi Hiraoka, Ling-Hui Zeng, David R Colman, Naomasa Miki; Cadherin activity is required for activity-induced spine remodeling . J Cell Biol 6 December 2004; 167 (5): 961–972. doi: https://doi.org/10.1083/jcb.200406030
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement