Septins (red) corral division proteins (Spa2; green) at the bud neck.

Barral/AAAS

Septin filaments in budding yeast make a corral that restricts certain division proteins to the site of cytokinesis, according to Jeroen Dobbelaere and Yves Barral (ETHZ, Zurich, Switzerland).

The corral is formed when the septin ring at the bud neck splits to form two parallel fences. The Swiss researchers had earlier noted that during this period septin becomes transiently mobile before reverting to a static organization.

They have now extended this investigation of mobility to other cytokinesis proteins. Three membrane-associated proteins involved in polarizing the actin cytoskeleton (Spa2), secretion (Sec3), and delivering cell wall to the division site (Chs2) were all extremely mobile at the division site, but did not diffuse to regions outside of the division site. Disruption of the septin barriers via a temperature-sensitive mutant led to rapid loss of the...

The Rockefeller University Press
2004
The Rockefeller University Press
2004
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