The dynamic regulation of polarized cell growth allows cells to form structures of defined size and shape. We have studied the regulation of polarized growth using mating yeast as a model. Haploid yeast cells treated with high concentration of pheromone form successive mating projections that initiate and terminate growth with regular periodicity. The mechanisms that control the frequency of growth initiation and termination under these conditions are not well understood. We found that the polarisome components Spa2, Pea2, and Bni1 and the Cdc42 regulators Cdc24 and Bem3 control the timing and frequency of projection formation. Loss of polarisome components and mutation of Cdc24 decrease the frequency of projection formation, while loss of Bem3 increases the frequency of projection formation. We found that polarisome components and the cell fusion proteins Fus1 and Fus2 are important for the termination of projection growth. Our results define the first molecular regulators that control the timing of growth initiation and termination during eukaryotic cell differentiation.
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19 January 2004
Article|
January 20 2004
Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators
Scott Bidlingmaier,
Scott Bidlingmaier
1Department of Molecular Biophysics and Biochemistry, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520
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Michael Snyder
Michael Snyder
1Department of Molecular Biophysics and Biochemistry, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520
2Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520
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Scott Bidlingmaier
1Department of Molecular Biophysics and Biochemistry, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520
Michael Snyder
1Department of Molecular Biophysics and Biochemistry, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520
2Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520
Address correspondence to Michael Snyder, Department of Molecular, Cellular, and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520-8103. Tel.: (203) 432-6139. Fax: (203) 432-3597. email: [email protected]
Abbreviations used in this paper: GAP, GTPase-activating protein; GEF, guanine nucleotide exchange factor; Lat-A, latrunculin A.
Received:
July 10 2003
Accepted:
December 04 2003
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2004
J Cell Biol (2004) 164 (2): 207–218.
Article history
Received:
July 10 2003
Accepted:
December 04 2003
Citation
Scott Bidlingmaier, Michael Snyder; Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators . J Cell Biol 19 January 2004; 164 (2): 207–218. doi: https://doi.org/10.1083/jcb.200307065
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