Cytoplasmic dynein has been implicated in numerous aspects of intracellular movement. We recently found dynein inhibitors to interfere with the reorientation of the microtubule cytoskeleton during healing of wounded NIH3T3 cell monolayers. We now find that dynein and its regulators dynactin and LIS1 localize to the leading cell cortex during this process. In the presence of serum, bright diffuse staining was observed in regions of active ruffling. This pattern was abolished by cytochalasin D, and was not observed in cells treated with lysophosphatidic acid, conditions which allow microtubule reorientation but not forward cell movement. Under the same conditions, using total internal reflection fluorescence microscopy, clear punctate dynein/dynactin containing structures were observed along the sides and at the tips of microtubules at the leading edge. Overexpression of dominant negative dynactin and LIS1 cDNAs or injection of antidynein antibody interfered with the rate of cell migration. Together, these results implicate a leading edge cortical pool of dynein in both early and persistent steps in directed cell movement.
Skip Nav Destination
Article navigation
22 December 2003
Report|
December 22 2003
A role for cytoplasmic dynein and LIS1 in directed cell movement
Denis L. Dujardin,
Denis L. Dujardin
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
2Ecole Supérieure de Biotechnologie de Strasbourg, Centre National de la Recherche Scientifique, UMR 7100, Parc d'Innovation, Boulevard Sébastien Brandt, BP 10413, 67412 ILLKIRCH Cedex, France
Search for other works by this author on:
Lora E. Barnhart,
Lora E. Barnhart
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
Search for other works by this author on:
Stephanie A. Stehman,
Stephanie A. Stehman
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
Search for other works by this author on:
Edgar R. Gomes,
Edgar R. Gomes
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
Search for other works by this author on:
Gregg G. Gundersen,
Gregg G. Gundersen
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
Search for other works by this author on:
Richard B. Vallee
Richard B. Vallee
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
Search for other works by this author on:
Denis L. Dujardin
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
2Ecole Supérieure de Biotechnologie de Strasbourg, Centre National de la Recherche Scientifique, UMR 7100, Parc d'Innovation, Boulevard Sébastien Brandt, BP 10413, 67412 ILLKIRCH Cedex, France
Lora E. Barnhart
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
Stephanie A. Stehman
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
Edgar R. Gomes
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
Gregg G. Gundersen
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
Richard B. Vallee
1College of Physicians and Surgeons, Department of Pathology, and Department of Anatomy and Cell Biology, Columbia University, New York, NY 10032
Address correspondence to Richard B. Vallee, Columbia University, College of Physicians and Surgeons, Dept. of Pathology and Anatomy and Cell Biology, P & S 15-409, 630 W. 168th St., New York, NY 10032. Tel.: (212) 342-0546. Fax: (212) 305-5498. email: [email protected]
Abbreviations used in this paper: LPA, lysophosphatidic acid; TIRF, total internal reflection fluorescence microscopy.
Received:
October 20 2003
Accepted:
November 13 2003
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2003
J Cell Biol (2003) 163 (6): 1205–1211.
Article history
Received:
October 20 2003
Accepted:
November 13 2003
Citation
Denis L. Dujardin, Lora E. Barnhart, Stephanie A. Stehman, Edgar R. Gomes, Gregg G. Gundersen, Richard B. Vallee; A role for cytoplasmic dynein and LIS1 in directed cell movement . J Cell Biol 22 December 2003; 163 (6): 1205–1211. doi: https://doi.org/10.1083/jcb.200310097
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionEmail alerts
Advertisement
Advertisement