tER sites (green) are disorganized in the absence of dp115 (right).

On page 185, Kondylis and Rabouille find that a protein required for Golgi structure also organizes transitional ER (tER) sites, also known as ER exit sites. Although addressing tER assembly, the results might also influence models of Golgi formation.

tER sites are enriched in COPII vesicles containing proteins destined for the Golgi. In fly S2 cells, tER sites are found adjacent to well-defined Golgi stacks. The authors have found that tER organization in S2 cells depends on dp115, of which mammalian homologues are necessary for vesicle transport and Golgi structure. In the absence of dp115, small tER sites that were no longer spatially associated with the Golgi were scattered throughout the cytoplasm.

As expected, Golgi stacks were fragmented into vesicles and tubules in dp115-depleted cells. Transport was not inhibited, however, so at least...

The Rockefeller University Press
2003
The Rockefeller University Press
2003
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