ELECTRON MICROSCOPIC AUTORADIOGRAPHY An Improved Technique for Producing Thin Films and Its Application to H3-Thymidine-Labeled Maize Nuclei

Koehler , J. K.; Mühlethaler , K.; Freywyssling , A.

doi:10.1083/jcb.16.1.73

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Article| January 01 1963

ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H³-Thymidine-Labeled Maize Nuclei

J. K. Koehler,

J. K. Koehler

From the Electron Microscope Division of the Institute for General Botany, Swiss Federal Institute of Technology, Zurich, Switzerland.

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K. Mühlethaler,

K. Mühlethaler

From the Electron Microscope Division of the Institute for General Botany, Swiss Federal Institute of Technology, Zurich, Switzerland.

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A. Freywyssling

From the Electron Microscope Division of the Institute for General Botany, Swiss Federal Institute of Technology, Zurich, Switzerland.

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Author and Article Information

J. K. Koehler

From the Electron Microscope Division of the Institute for General Botany, Swiss Federal Institute of Technology, Zurich, Switzerland.

K. Mühlethaler

From the Electron Microscope Division of the Institute for General Botany, Swiss Federal Institute of Technology, Zurich, Switzerland.

A. Freywyssling

From the Electron Microscope Division of the Institute for General Botany, Swiss Federal Institute of Technology, Zurich, Switzerland.

Dr. Koehler's present address is Department of Physics, New Mexico Highlands University, Las Vegas, New Mexico

Received: May 25 1962

Online ISSN: 1540-8140

Print ISSN: 0021-9525

Copyright, 1963, by The Rockefeller Institute Press

1963

J Cell Biol (1963) 16 (1): 73–80.

https://doi.org/10.1083/jcb.16.1.73

An improved method has been developed for the preparation of autoradiographs for electron optical study. The refinement lies principally in the routine production of uniformly thin layers of photographic emulsion over the tracer-labeled cell sections. This is accomplished by means of a centrifugal spreading mechanism. Maize root tips grown in the presence of H³-thymidine were examined electron microscopically by this technique and were found to display nuclear labeling with impressive clarity. The new procedure utilized here yields objects in which the finest cell structures are easily resolvable without recourse to gelatin digestion techniques.

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Copyright, 1963, by The Rockefeller Institute Press

1963

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ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H3-Thymidine-Labeled Maize Nuclei

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ELECTRON MICROSCOPIC AUTORADIOGRAPHY : An Improved Technique for Producing Thin Films and Its Application to H³-Thymidine-Labeled Maize Nuclei