Transformation of a contact between axon and dendrite into a synapse is accompanied by accumulation of the synaptic machinery at this site, being delivered in intracellular organelles mainly of TGN origin. Here, we report that in cultured hippocampal neurons, TGN organelles are linked via spectrin to clusters of the neural cell adhesion molecule (NCAM) in the plasma membrane. These complexes are translocated along neurites and trapped at sites of initial neurite-to-neurite contacts within several minutes after initial contact formation. The accumulation of TGN organelles at contacts with NCAM-deficient neurons is reduced when compared with wild-type cells, suggesting that NCAM mediates the anchoring of intracellular organelles in nascent synapses.
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25 November 2002
Article|
November 18 2002
Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts
Vladimir Sytnyk,
Vladimir Sytnyk
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
2Laboratory for Biophysics and Bioelectronics, Dnepropetrovsk State University, 49050 Dnepropetrovsk, Ukraine
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Iryna Leshchyns'ka,
Iryna Leshchyns'ka
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
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Markus Delling,
Markus Delling
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
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Galina Dityateva,
Galina Dityateva
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
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Alexander Dityatev,
Alexander Dityatev
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
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Melitta Schachner
Melitta Schachner
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
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Vladimir Sytnyk
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
2Laboratory for Biophysics and Bioelectronics, Dnepropetrovsk State University, 49050 Dnepropetrovsk, Ukraine
Iryna Leshchyns'ka
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
Markus Delling
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
Galina Dityateva
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
Alexander Dityatev
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
Melitta Schachner
1Zentrum für Molekulare Neurobiologie, Universität Hamburg, D-20246 Hamburg, Germany
Address correspondence to Melitta Schachner, Zentrum für Molekulare Neurobiologie, Universität Hamburg, Martinistrasse 52, D-20246 Hamburg, Germany. Tel.: 49-40-42803-6246. Fax: 49-40-42803-6248. E-mail: [email protected]
The online version of this article includes supplemental material.
*
Abbreviations used in this paper: DIC, differential interference contrast; NCAM, neural cell adhesion molecule.
Received:
May 20 2002
Revision Received:
September 24 2002
Accepted:
October 14 2002
Online ISSN: 1540-8140
Print ISSN: 0021-9525
The Rockefeller University Press
2002
J Cell Biol (2002) 159 (4): 649–661.
Article history
Received:
May 20 2002
Revision Received:
September 24 2002
Accepted:
October 14 2002
Citation
Vladimir Sytnyk, Iryna Leshchyns'ka, Markus Delling, Galina Dityateva, Alexander Dityatev, Melitta Schachner; Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts . J Cell Biol 25 November 2002; 159 (4): 649–661. doi: https://doi.org/10.1083/jcb.200205098
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